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| 重组大鼠胰岛素样生长因子-1聚(乳酸-羟基乙酸)共聚物缓释微球对2型糖尿病GK大鼠种植体周围骨形成的影响 | |
| 引用本文： | 于淼,惠新民,王峰,张宁,张和鹏,宋应亮.重组大鼠胰岛素样生长因子-1聚(乳酸-羟基乙酸)共聚物缓释微球对2型糖尿病GK大鼠种植体周围骨形成的影响[J].中国医学科学院学报,2010,32(3). |
| 作者姓名： | 于淼 惠新民 王峰 张宁 张和鹏 宋应亮 |
| 作者单位： | 1. 第四军医大学口腔医学院种植科,西安,710032 2. 陕西省黄陵县人民医院口腔科,陕西黄陵,727300 3. 第四军医大学病原生物学教研室,西安,710032 4. 西北工业大学理学院应用化学系,西安,710072 |
| 摘 要： | 目的 评估重组大鼠胰岛素样生长因子-1(rrIGF-1)聚(乳酸-羟基乙酸)共聚物(PLGA)缓释微球对2型糖尿病GK大鼠种植体周围骨形成的影响.方法 将20只雄性GK大鼠进行糖尿病造模成功后随机分为2组,分别给予加载包裹rrIGF-1的PLGA缓释微球(治疗组,n=10)和加载包裹与rrIGF-1等量安慰剂的PLGA缓释微球(糖尿病组,n=10)处理,再以10只未做处理的SD大鼠作为对照组.在所有大鼠胫骨内植人种植体,治疗组和糖尿病组大鼠同时分别加载等量相应种类的PLGA缓释微球.术后4、5、8周在种植体周围局部采血,采用酶联免疫吸附法(ELISA)检测血清骨钙素(OCN)、骨特异性碱性磷酸酶(B-ALP)及Ⅰ型前胶原羧基前肽(PICP)含量.结果 种植体植入术后4周,糖尿病组和治疗组的血清OCN、B-ALP和PICP水平均明显低于对照组(P均＜0.05);术后5周,糖尿病组的OCN和B-ALP水平明显低于对照组和治疗组(P均＜0.05),糖尿病组和治疗组的PICP水平也均明显低于对照组(P＜0.05),治疗组的OCN水平明显高于术后4周(P＜0.05),对照组的PICP水平则明显低于术后4周(P＜0.05);术后8周,糖尿病组大鼠的OCN和B-ALP水平明显低于对照组和治疗组(P均＜0.05),糖尿病组和治疗组大鼠的血清PICP水平均明显低于对照组(P均＜0.05).结论 rrIGF-1 PLGA缓释微球局部加载可促进GK大鼠种植体周围骨形成. |
| 关 键 词： | 重组大鼠胰岛素样生长因子-1 聚(乳酸-羟基乙酸)共聚物 2型糖尿病 GK大鼠 骨钙素 骨特异性碱性磷酸酶 Ⅰ型前胶原羧基前肽 |
Effect of Sustained Release of Recombinant Rat Insulin-like Growth Factor-1 from Poly (lactide-CO-glycolide) Microspheres on Bone Formation in the Peri-implant Areas in Goto-Kakizaki Rats with Type 2 Diabetes |
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| YU Miao,HUI Xin-min,WANG Feng,ZHANG Ning,ZHANG He-peng,SONG Ying-liang.Effect of Sustained Release of Recombinant Rat Insulin-like Growth Factor-1 from Poly (lactide-CO-glycolide) Microspheres on Bone Formation in the Peri-implant Areas in Goto-Kakizaki Rats with Type 2 Diabetes[J].Acta Academiae Medicinae Sinicae,2010,32(3). | |
| Authors: | YU Miao HUI Xin-min WANG Feng ZHANG Ning ZHANG He-peng SONG Ying-liang |
| Institution: | YU Miao1,HUI Xin-min2,WANG Feng1,ZHANG Ning3,ZHANG He-peng4,SONG Ying-liang11Department of Oral Implantology,School of Stomatology,Fourth Military Medical University,Xi'an 710032,China2Department of Stomatology,People's Hospital of Huangling County,Huangling County,Shaanxi Province 727300,China3Department of Pathogenic Biology,China4Department of Applied Chemistry,School of Science,Northwestern Polytechnical University,Xi'an 710072,China |
| Abstract: | Objective To evaluate the effect of sustained release of recombinant rat insulin-like growth factor-1(rrIGF-1) from poly(lactide-CO-glycolide)(PLGA) microspheres on bone formation in the peri-implant areas in Goto-Kakizaki rats with type 2 diabetes.Methods Type 2 diabetes models were successfully established in 20 male Goto-Kakizaki rats,which were then randomly divided into treatment group(sustained release of rrIGF-1 from PLGA microspheres were loaded on the peri-implant areas,n=10) and diabetic group(loa... |
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