| SPA-ELISA检测弓形虫感染鼠尿中的循环抗原 |
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| 引用本文： | 金武官,李云珠,俞善昌,杨惠珍. SPA-ELISA检测弓形虫感染鼠尿中的循环抗原[J]. 中国寄生虫学与寄生虫病杂志, 2000, 18(1): 43-45 |
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| 作者姓名： | 金武官 李云珠 俞善昌 杨惠珍 |
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| 作者单位： | 1. 上海第二医科大学附属瑞金医院儿科,上海,200025
2. 上海第二医科大学寄生虫教研组,上海,200025 |
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| 基金项目： | 国家自然科学基金资助项目(No．39070754) |
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| 摘 要： | [目的 ]探索弓形虫感染早期诊断的简便方法。 [方法 ]用SPA ELISA检测轻度、中度和重度感染弓形虫鼠尿中弓形虫循环抗原 (TCA)。 [结果 ]轻度、中度和重度感染弓形虫 3组鼠尿中均检出TCA ,上述 3组分别于弓形虫感染后d6 、d3和d4显示阳性斑点。 [结论 ]SPA ELISA能检出弓形虫感染鼠尿中的弓形虫循环抗原 ,可用于弓形虫感染的早期诊断。
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| 关 键 词： | 弓形虫 循环抗原 SPAELISA 鼠尿 |
| 文章编号： | 1000-7423(2000)-01-0043-03 |
| 修稿时间： | 1999-09-16 |
DETECTON OF CIRCULATING ANTIGEN IN URINE FROM MICE INFECTED WITH TOXOPLASMA TACHYZOITES BY SPA-ELISA |
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| JIN Wu-guan,LI Yun-zhu,YU Shan-cang,YANG Hui-zheng. DETECTON OF CIRCULATING ANTIGEN IN URINE FROM MICE INFECTED WITH TOXOPLASMA TACHYZOITES BY SPA-ELISA[J]. Chinese Journal of Parasitology and Parasitic Diseases, 2000, 18(1): 43-45 |
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| Authors: | JIN Wu-guan LI Yun-zhu YU Shan-cang YANG Hui-zheng |
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| Affiliation: | JIN Wu-guan,LI Yun-zhu,YU Shan-cang(Department of pediatric Medicine, Ruijin Hospital,Shanghai Second Medical University,Shanghai 200025)YANG Hui-zheng(Department of Parasitology,Shanghai Second Medical University,Shanghai 200025) |
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| Abstract: | Objective] To explore a simple and convenient immunoassay for early diagnosis of Toxoplasma infection.[Methods] Urine samples collected from three groups of mice infected with different doses of tachyzoites were detected for Toxoplasma circulating antigen (TCA) by dot ELISA using HRP SPA as a second antibody ( SPA ELISA).[Results] Toxoplasma circulating antigens were detected in all three groups of infected mice in contrast with the normal control group. Toxoplasma circulating antigen was detected on d 6 and d 3 after infection in mice of light and moderate infection groups, respectively.[Conclusion]SPA ELISA is a simple and convenient method for early immunodiagnosis of recent Toxoplasma infection. |
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| Keywords: | Toxoplasma circulating antigen SPA-ELISA mouse urine. |
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