脂质体包裹CpGODN和肿瘤抗原治疗肝癌的免疫效应

目的探索脂质体、CpG寡核苷酸（ODN）以及Hepa1-6肝癌细胞裂解物联合应用治疗肝癌的免疫效果。方法建立C57BL／6小鼠肝癌模型，以PBS、ODN＋rtep（肝癌细胞Hep）,Lip（Lipofectamine脂质体）＋HDN＋Hep荷瘤鼠背部皮下注射，各组小鼠肝脏HE染色，FCM检测小鼠脾脏CD3-FITC、CD4-FITC、CD8-FITC、NKl．1-FITC的组成，ELISA法测免疫小鼠血清中IFN-Y、IL-4水平。结果脂质体包裹CpGODN＋Hepa1-6肝癌细胞裂解物中CD3-FITC、CD4-FITC、CD8-F1TC、NKl．1-FITC的体外杀伤活性以及荷瘤小鼠血清中IFN-7、IL-4水平均显著高于CpGODN＋Hep组以及PBS组。结论脂质体包裹CpGODN＋Hep能显著提高机体的免疫功能，尤其是特异性细胞免疫功能。

Immune Effect of Liposomes Coating CpG ODN and Tumor Antigen for Liver Cancer Treatment

Objectives This article discussed the immune effect of combination application of liposomal,CpG oligodeoxynucleotide(ODN) and Hepa 1-6 hepatoma cell lysates for liver cancer treatment.Methods Liver cancer model of C57BL/6 mice was set up.Tumor-bearing mice were subcutaneously injected PBS,ODN + Hep,Lip + ODN + Hep treatment and observation.Liver of different group mice used HE staining.FCM detected the formation of spleen CD3-FITC,CD4-FITC,CD8-FITC,NK1.1-FITC.The serum of mice immunized with IFN-γ,I L-4 level was measured by ELISA method.Results The destruction to competence in vitro of liposomes coating CpG ODN + Hepa 1-6 and CD3-FITC、CD4-FITC 、CD8-FITC、NK1.1-FITC in lysate of liver cancer cells and level of IFN-γ、IL-4 in mice serum are much higher than in CpG ODN + Hep group and PBS group.Conclusions Liposomal CpG ODN and Hep can significantly improve immune function,especially the specific cellular immune function.