| 登革病毒cDNA的生物信息学分析及其O1igo探针设计 |
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| 引用本文： | 肖维威,马文丽,马晓冬,毛向明,郑文岭.登革病毒cDNA的生物信息学分析及其O1igo探针设计[J].第一军医大学学报,2003,23(9):905-907. |
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| 作者姓名： | 肖维威 马文丽 马晓冬 毛向明 郑文岭 |
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| 作者单位： | [1]第一军医大学分子生物学研究所,广东广州510515 [2]广州军区广州总医院分子肿瘤学研究所，广东广州510010,广东广州510515 |
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| 摘 要： | 目的 设计登革病毒诊断芯片的Oligo探针。方法 利用BLAST软件对4型登革病毒的cDNA序列进行序列比对，得到有意义的特异序列；然后用生物学软件Oligo6．0设计特异性高、Tm值相近、长度均一的Oligo探针。结果 获得48条70-mer Oligo探针，用于打印成DNA芯片，进行登革病毒的检测。结论 利用BLAST系统和生物学软件Olig06．0设计登革病毒诊断芯片的探针，是一种简便可行、有效的方法。
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| 关 键 词： | 登革病毒 cDNA 生物信息学 O1igo探针 生物芯片 BLAST软件 |
Bioinformatic analysis of dengue virus cDNAs and design of oligonucleotide probes for microarray detection of the virus] |
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| Wei-wei Xiao,Wen-li Ma,Xiao-dong Ma,Xiang-ming Mao,Wen-ling Zheng.Bioinformatic analysis of dengue virus cDNAs and design of oligonucleotide probes for microarray detection of the virus][J].Journal of First Military Medical University,2003,23(9):905-907. |
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| Authors: | Wei-wei Xiao Wen-li Ma Xiao-dong Ma Xiang-ming Mao Wen-ling Zheng |
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| Institution: | Institute of Molecular Biology, First Military Medical University, Guangzhou 510515,China. |
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| Abstract: | OBJECTIVE: To design oligonucleotide probes for microarray detection of dengue virus. METHODS: By analyzing the cDNAs of dengue viruses of 4 different serotypes with BLAST program, a group of specific sequences for the candidate probes was acquired. Oligo6.0 software was applied to analyze the candidates to select the probes with high specificity, identical length and similar melting temperature (Tm). RESULT: Altogether 48 oligonucleotide probes were designed, and deposited on oligonucleotide chips as the microarray for dengue virus detection. CONCLUSION: BLAST program and Oligo6.0 software are simple and effective means for designing the oligonucleotide probes. |
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