子宫内膜异位症体外组织学模型的构建

目的:分析子宫内膜与腹膜黏附的细胞学行为及分子生物学表现,探索子宫内膜组织培养条件及子宫内膜组织与腹膜组织黏附的培养条件。方法:对比观察气液面培养和培养基中培养对于子宫内膜组织及子宫内膜组织黏附腹膜的影响,子宫内膜黏附腹膜后分别于1h,6h,12h,24h,1h～6天收集标本用光镜观察子宫内膜与腹膜黏附发生的时间,黏附部位的细胞学行为。结果:子宫内膜组织气液平面培养较培养基中培养组织结构维持好,离体培养时间长;子宫内膜组织接种腹膜组织离体培养3d后能观察到子宫内膜基质细胞明显侵袭腹膜间皮层,黏附处大部分腹膜间皮层缺损,可以通过腺体和基质细胞与腹膜间皮面黏附。结论:子宫内膜组织与腹膜组织共培养于气液平面培养效果好。

Establishment of a novel in vitro histological model for human endometriosis.

Objective:To investigate cultural environment for endometrium and its adhesion to peritoneum and analyze the cellular and molecular feature of the adhesion.Methods:We observed and compared the effects of gas-liquid surface culture and in-medium culture on the growth of endometrium and the adhesion of endometrium to peritoneum.Collect specimens at 1h,6h,12h,24h,1h~6d for observation under light microscope and electron microscope to learn the timing of adhesion and the cellular features at adhesion sites.Results:Gas-liquid surface culture was superior to in-medium culture in maintaining tissue structure and survival.Faster adhesion and invasion of endometrium to peritoneum is demonstrated in gas-liquid surface culture.Peritoneal mesothelium defects were found after adhesion by gland cells and stromal cells of endometrium and disappeared gradually as the cultivation continued.Apparent invasion of endometrium into peritoneum was observed in culture 3 days after inoculation.Conclusion:Gas-liquid surface culture is a suitable model for research on human endometrium culture and its adhesion to peritoneum.