Blocking of human fertilization in vitro by sera with sperm-immobilizing antibodies |
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Authors: | M Kamada T Daitoh H Hasebe M Irahara S Yamano T Mori |
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Institution: | 1. Department of Obstetrics and Gynecology, School of Medicine, University of Tokushima, Tokushima, Japan;2. Department of Gynecology and Obstetrics, Faculty of Medicine, Kyoto University, Kyoto, Japan |
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Abstract: | Serum samples with sperm-immobilizing antibody activity from six women were examined for ability to block sperm-egg interaction by a zona penetration test where human follicular ova matured in vitro were used. Exposure of spermatozoa from a fertile healthy donor to the sera impaired binding to and penetration through the zona pellucida of the spermatozoa completely in five cases and incompletely in one case. Successful fertilization in vitro was achieved by using fetal cord serum instead of autoserum of the patient included in the in vitro fertilization and embryo transfer program. These results suggest that interference with sperm-egg interaction may be an additional mechanism of infertility that is caused by antisperm antibodies. |
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Keywords: | Antisperm antibodies human zona pellucida sperm penetration test in vitro fertilization |
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