硒碘缺乏对大鼠空间学习记忆能力的影响

目的探讨硒、碘缺乏对连续繁殖的三代生长发育期的SD大鼠空间学习记忆能力的影响。方法实验分为对照组、低硒组、低碘组、低硒低碘组,通过测定大鼠在Morris水迷宫中的各种参数来反映其空间学习记忆能力。结果仔一代和仔二代大鼠在定位航行实验和空间探索实验各参数差异均无统计学意义,仔三代大鼠在定位航行实验中,低硒低碘组雌鼠9个时段的平均潜伏期比对照组明显延长。在空间探索实验中,雌鼠在各象限停留时间各组之间差异无统计学意义,但对照组在目标象限停留时间较其他3个组长,在远离目标象限停留时间短。低硒低碘组雌鼠穿过原平台所在位置的次数较对照组明显减少。结论硒、碘缺乏对仔一代和仔二代生长发育期大鼠的空间学习记忆能力没有明显影响;单纯的膳食性低硒、低碘对仔三代大鼠空间学习记忆能力没有明显影响,但硒、碘水平同时降低却可以明显降低空间学习记忆能力,而且硒、碘水平同时降低对雌性大鼠学习记忆的影响更为显著。

Effect of selenium and(or) iodine deficiency on the ability of spatial learning and memory of rats in developing stage

Objective To explore the effect of selenium and(or) iodinedeficiencyontheabilityofspatiallearningand memory of successively bred rats in developing stage. Method The selenium and(or) iodine deficiency rat modelwas established by feeding the selenium and/or iodine deficient food. The rats were divided into 4 groups: controlsupplemented with both selenium and iodine (Se I ), selenium deficient group supplemented with iodine only(Se- I ),iodine deficiency group supplemented with selenium only (Se I-) and both deficient group supplemented with neitherselenium nor iodine (Se- I- ). The parameters of rats in the Morris water maze were investigated. Result In theorientating navigation test, for the F3 female rats the average latencies in Se- I- group were significantly longer thanthat of control group (P < 0.05); In the spatial probe test there were no significant differences of time staying indifferent quadrants among four groups of both male and female F3 rats. But the female rats of control group spent moretime in target quadrant than the other three groups did. There were no significant differences of the times passingthrough the platform for the male rats of four groups, but the times for the female rats of Se- plus I- group significantlydecreased. There were no significant differences of the parameters in both orientating navigation test and spatial probetest of F1 and F2 rats. Conclusion Selenium and (or) iodine deficiency have no significant effects on the learning andmemory of F1 and F2 rats in developing stage; selenium or iodine deficiency had no significant effect on the spatiallearning and memory of F3 rats. However, a combination of selenium and iodine deficiency demonstrates mostsignificant effects on the spatial learning and memory, especially for the female F3 rats.