成纤维细胞在聚羟基丁酸酯表面黏附与种植研究

可降解高分子聚合物广泛应用于组织工程，其表面的黏附特性影响细胞的种植与生长。我们研究了成纤维细胞系NIH3T3在可降解材料聚羟基丁酸酯（PHB）表面的黏附和生长。应用线性变剪切流槽研究NIH3T3细胞在材料表面的黏附力学特性，结果表明，由于在PHB表面的临界脱离剪应力较低，高密度接种时间细胞趋于聚集；在经多聚赖氨酸衣被的表面临界脱离剪应力提高，细胞铺展生长。对三维PHB多孔支架种植细胞实验表明，在PHB材料表面进行多聚赖氨酸衣被，可以有效地提高细胞种植效率。

The Adhesion and Seeding of NIH3T3 Fibroblast on PHB Surfaces

Biodegradable polymers have been widely used in the field of tissue engineering. The adhesive characteristic of polymer surfaces is critical for cell seeding and growth. The adhesion and growth of NIH3T3 fibroblast on poly-beta-hydroxybutrpic acid (PHB) was studied here, and the cell-surface adhesion strength was measured in a shear flow detachment assay. It showed that the critical shear stress for cell detachment was relatively low on PHB, thus cells tend to aggregate and detach from surfaces. On the other hand, cells tend to spread and formed monolayer on poly-lysine coated PHB surface. When the PHB foams scaffolds were coated by poly-lysine, the seeding percentage of cells was also greatly enhanced.