Kinetic analysis of the humoral immune response against 3 Toxoplasma gondii-recombinant proteins in infants with suspected congenital toxoplasmosis |
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| Authors: | Altcheh Jaime Diaz Natalia S Pepe Carolina M Martin Valentina Nigro Monica Freilij Hector Angel Sergio O |
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| Affiliation: | 1. Parasitología, Hospital de Niños Ricardo Gutierrez, Buenos Aires 1429, Argentina;2. Dto. de Parasitología, INEI, ANLIS Dr Carlos G. Malbran, Buenos Aires 1281, Argentina;3. Laboratorio de Parasitología Molecular, IIB-INTECH, CONICET-UNSAM, Chascomús, Provincia de Buenos Aires 7130, Argentina;1. Aix Marseille Univ, IRD, AP-HM, MEPHI, Marseille, France;2. Leptospirosis Laboratory, VetAgro Sup, Veterinary Campus of Lyon, Marcy l’Etoile, France;3. IHU Méditerranée Infection, Marseille, France;4. Aix Marseille Univ, IRD, AP-HM, SSA, VITROME, Marseille, France;1. Department of Mathematics, Tongji University, Shanghai 200092, PR China;2. Department of Applied Mathematics, University of Western Ontario, London, Ontario N6A 5B7, Canada;1. School of Agriculture, Ludong University, Yantai, China;2. Laboratory of Marine Biology and Biotechnology, Qingdao National Laboratory for Marine Science and Technology, Qingdao, China;3. Laboratory of Fish Molecular Immunology, College of Fisheries and Life Science, Shanghai Ocean University, Shanghai, China;1. Department of Pathology, Case Western Reserve University, 11100 Euclid Av., Cleveland, OH, 44106, USA;2. Division of Infectious Diseases and HIV Medicine, Dept. of Medicine, Case Western Reserve University, 11100 Euclid Av., Cleveland, OH, 44106, USA;3. Department of Ophthalmology and Visual Sciences, Case Western Reserve University, 11100 Euclid Av., Cleveland, OH, 44106, USA;1. Anhui Provincial Laboratory of Microbiology and Parasitology, Anhui Key Laboratory of Zoonoses, Department of Parasitology, Anhui Medical University, Hefei 230022, PR China;2. Department of Immunology, Anhui Medical Iniversity, Hefei 230022, PR China;3. The Central Laboratory of Affiliated Provincial Hospital of Anhui Medical University, Hefei 230022, PR China;1. Institute of Pathogenic Biology, Medical College, University of South China, Hunan Provincial Key Laboratory for Special Pathogens Prevention and Control, Hengyang 421001, China;2. Department of Laboratory Medicine, The Second Affiliated Hospital of University of South China, Hengyang 421001, China;3. Hunan Province Cooperative Innovation Center for Molecular Target New Drug Study, Hengyang 421001, China |
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| Abstract: | The reactivity values of Toxoplasma gondii ROP2, GRA4, and GRA7 recombinant antigens (rAgs) were analyzed by immunoglobulin G (IgG) enzyme-linked immunosorbent assay (ELISA) in 23 congenitally infected (I) and 36 noninfected (NI) infants. The reactivity values observed from the serum samples of I versus NI infants for each recombinant protein were 91% versus 67% for rROP2 (P = .05), 86% versus 29% for rGRA4 (P < .001), and 56% versus 11% for rGRA7 (P = .003). The follow-up showed that serum samples from NI infants became negative for specific IgG at 5.8 months (95% confidence interval [CI], 4.9-6.7) using a commercial assay; meanwhile, by specific recombinant protein ELISA, the samples became negative at 3.7 months with rROP2 (95% CI, 2.8-4.6), at 1.3 months with rGRA4 (95% CI, 0.8-1.8), and at 0.9 months with rGRA7 (95% CI, 0.5-1.3). Kinetic analysis also showed that serum samples from group I presented different IgG-profiles among rAgs. The rROP2 IgG profile was similar to that of the commercial assay, whereas rGRA4 and rGRA7 profiles showed a gradual decrease along the period of the study. The potential of the utility of rAgs to develop a diagnostic system that discriminates congenitally I infants from NI is discussed. |
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