酒精性肝损伤大鼠肠道屏障功能改变及丹参的保护作用

目的]观察酒精性肝损伤大鼠肠道屏障功能（IBF）的改变，并探讨丹参对IBF的保护作用。方法]36只SD大鼠随机分为正常对照（A）组、模型（B）组、丹参治疗（C）组，每组12只，分别于实验12周末处死动物，检测肠道细菌易位率、肠黏膜通透性，同时观察肝脏生化、肝脏和末段回肠黏膜病理学改变。结果]大鼠血清丙氨酸氨基转移酶、天冬氨酸转氨酶、肠道细菌易位率B组显著高于A、C组（均P〈0．05），A、C组之间差异无统计学意义（P〉0．05）；肠黏膜通透性A组显著低于B、C组（均P〈0．05），C组显著低于B组（P〈0．05）；末段回肠病理损伤B组显著重于A、C组（均P〈0．05），C组显著重于A组（P〈0．05）。结论]大鼠酒精性肝损伤伴有IBF改变，丹参对大鼠酒精性肝损伤及IBF具有双重保护作用。

Experimental study on the changes in intestinal barrier function and protection of Salvia Miltiorrhiza on ethanol-induced liver injury in rats

Objective]To investigate the changes in intestinal barrier function（IBF）and protection of Salvia Miltiorrhiza on ethanol-induced liver injury in rats. Methods] Thirty six SD rats were divided into Group A（normal control group） , Group B（model group）and Group C（Salvia Miltiorrhiza treat group）randomly with 12 rats in each group. In the 12th week the rats were sacrificed, then the changes of ALT and AST was detected. The rate of bacterial transloeation（BT） was calculated, the intestinal mucosal permeability was detected, the histopathologic changes of the liver and terminal ileum were examination. Results] （1）The level of AST and ALT of Group B increased obviously compared with that of Group A and C（P〈0. 05）, but the level of AST and ALT of Group A and C had no significant difference（P〈0. 05）. （2）Intestinal mueosal permeability in Group B,C was more significant than Group A,Group B was more significant than Group C （P〈0. 05）. （3）The rate of intestinal tract BT in Group B,C was higher than that of Group A, Group B was more significant than Group C（P〈0. 05）,Group A and C had no significant difference（P〈0. 05）. （4）The intestinal mucosa injuring pathological changes in Group B was more significant than Group A, C, Group C was more significant than Group A（P〈0. 05）. Conclusion]In experimental ethanol-induced liver injury was accompanied by IBF injury at the same time. Salvia Miltiorrhiza has double protection of ethanol induced liver and IBF.