二氢卟吩e6脂质体的制备及影响因素考察

目的:建立二氢卟吩e6脂质体包封率的测定方法,考察各因素对包封率的影响.方法:分别采用Sephadex G50,Sephadex G75凝胶柱分离脂质体和游离药物,优选测定二氢卟吩e6脂质体包封率的方法；以包封率为指标,采用单因素试验考察磷脂质量浓度、胆固醇用量、水化介质、温度及时间对二氢卟吩e6脂质体制备的影响.结果:选择SephadexG50柱色谱法测定二氢卟吩e6的包封率,其对游离药物回收率达100.6％,采用该法测定包封率的重复性RSD＜0.02；磷脂与胆固醇的质量比、药脂比、水化介质对包封率的影响较大.结论:SephadexG50凝胶柱色谱法能方便、快速地测定二氢卟吩e6脂质体的包封率,建立的方法稳定可行.

Preparation and Factors Investigation Affecting Entrapment Efficiency of Chlorin e6 Liposomes

Objective:To establish a method for determining entrapment efficiency (EE) of chlorin e6 liposomes,and to investigate effect of different factors on it. Method: Sephadex G50 and Sephadex G75 column filtration methods were used to separate free drug and liposomes,in order to optimize a method to determine EE of chlorin e6 liposomes;With EE as index,influence of phospholipid concentration,cholesterol amount,hydration medium,temperature and time on preparation of chlorin e6 liposomes were investigated by single factor test. Result: EE of chlorin e6 liposomes was determined by Sephadex G50 column chromatography,its recovery for free drug was up to 100.6%,RSD of repeatability test was less than 0.02;Ratio of phospholipid-cholesterol,drug-lipid ratio and hydration medium had great effect on EE. Conclusion: Sephadex G50 column filtration method was a good way to determine EE of chlorin e6 liposomes easily and quickly,established method was stable and feasible.