藤黄炮制品对大鼠肠道组织病理学研究

目的:观察藤黄各制剂单次大剂量ig给药后大鼠消化系统病变情况,并比较藤黄炮制前后对大鼠肠上皮细胞株IEC-6毒性差异,探讨藤黄炮制减毒机制.方法:单次ig给予藤黄生品(200 mg· kg-1)、清水制藤黄(200 mg· kg-1)和藤黄酸(50 mg· kg-1),观察给药后大鼠的活动情况,于24 h后处死并采集大鼠心、肝、肾、食管、胃、十二指肠、空肠、回肠和结肠等主要脏器,观察大鼠口服给药后消化系统病变情况.以大鼠肠上皮细胞株IEC-6为研究对象,采用MTT法检测藤黄炮制前后对IEC-6细胞增殖的影响,采用倒置显微镜观察细胞形态.结果:病理切片结果显示,给药后大鼠的肝脏、肾脏、心脏、胃和食道均未见明显的毒性反应；但十二指肠、空肠有轻度至重度的毒性作用,表现为肠绒毛水肿.3种藤黄制剂均会引起给药大鼠肠道的毒性作用,生品藤黄所致的十二指肠和空肠水肿程度最严重,其次是清水制藤黄和藤黄酸.细胞的毒性结果显示,与空白组相比,藤黄生品及炮制品均可明显降低IEC-6细胞的活性(P＜0.01)与形态,且呈一定的剂量相关性；与藤黄生品组相比,藤黄3种炮制品组对IEC-6细胞的增殖抑制作用(P＜0.01)和形态变差的趋势均显著性降低.结论:藤黄单次大剂量ig给药后大鼠的病理变化及IEC-6细胞的毒性作用结果表明,藤黄经炮制后毒性显著降低.

Pathological Study on Toxicity of Gamboges in vivo and in vitro

Objective:To observe digestive system disease of rats after oral administration by Gamboges,compare the toxicity on rat intestinal epithelial cell line IEC-6 of the gamboges and the processed products and discuss the possible mechanism of detoxication. Method: Single administration of the gamboges, the gamboges processed with water and the gambogic acid was carried out, and the heart, liver, kidney, esophagus, stomach, duodenum, jejunum, ileum and colon were collected to observe digestive system change. The method of MTT was adopted to detect the cell proliferation of intestinal epithelial cell line IEC-6; the shapes of cells were observed by inverted microscope. Result: The results of the pathological section showed that there was no visible toxicity in the liver, kidney, heart, stomach and esophagus. But there was toxicity in duodenum and jejunum, showed the intestinal villus dropsical. All the experimental drugs could cause the toxicities in intestinal tract, and the gamboges were harmer than the other two. Cell toxicity results showed that the gamboges could obviously inhibit the cell proliferation when compared with control group (P<0.01), the cell toxicities of produced products were obviously lower than gamboges (P<0.01). Conclusion: The results of the pathological section and the cell toxicity both showed that the toxicity of gamboges was obviously reduced after processing, which provides a basis for further exploration of detoxication mechanism of gamboges and the processed products.