川芎中总荧光物质的含量测定及提取工艺优化

目的:建立川芎中总荧光物质的含量测定方法,优化总荧光物质的超声提取工艺.方法:采用荧光分光光度法测定川芎中总荧光物质的含量.通过单因素试验考察料液比、超声时间、提取溶剂种类与浓度对总荧光物质提取工艺的影响,采用星点设计对总荧光物质超声提取工艺的参数进行优选,通过对自变量各水平的多元线性回归及二项式拟合,选用效应面法选取最佳工艺,并进行预测分析.结果:确定的最佳工艺条件为乙醇体积分数63％,液固比350∶1,超声53 min.川芎中总荧光物质提取率的预测值达3.444％,预测值与验证值偏差2.70％.二项式拟合R2=0.9532.结论:优选的提取工艺具有简便、精密度高、可预测性好的优点；建立的含量测定方法稳定可行,为川芎药材的质量控制提供理论依据.

Content Determination and Extraction Technology Optimization of Total Fluorescent Substances from Ligusticum chuanxiong

Objective:To establish content determination method of total fluorescent substances from Ligusticum chuanxiong,and optimize ultrasonic extraction technology of total fluorescent substances. Method: Fluorescence spectrophotometric method was adopted for determining the content of total fluorescent substances from L. chuanxiong.Influence of solid-liquid ratio,extraction time,kind and concentration of extraction solvent on extraction technology of total fluorescent substances was investigated by single factor test,ultrasound extraction technology parameters of total fluorescent substances were optimized by composite design,multiple linear regression and binomial fitting were used to evaluate relationship between independent and dependent variables,response surface methodology was used to optimize extraction process,prediction analysis was carried out by comparing the observed value and the predicted value. Result: Optimal technology conditions of ultrasonic extraction process were as followings:ultrasound extracted 53 min with 350 times the amount of 63% ethanol.The predictive extraction rate of total fluorescent substances from L. chuanxiong was 3.444%,bias between the observed value and the predicted value was 2.70%.Regression coefficients of binomial fitting complex model was as high as 0.953 2. Conclusion: This optimized ultrasonic extraction technology was simple with high precision and good predictability.Established content determination method was stable and feasible,it could provide a theoretical basis for quality control of L. chuanxiong.