高效液相色谱法测定胆汁中头孢美唑

开发一个可用于定量测定胆汁中头孢美唑浓度的高效液相色谱分析方法。方法：取０．２ｍＬ胆汁用水稀释至１ｍＬ，并转移至已准备好的Ｃ１８固相萃取柱中进行固相萃取。用１ｍＬ１０％乙腈水溶液洗脱吸附在固相萃取柱上的头孢美唑，并收集于样品瓶中用高效液相色谱法进行分析测定。流劝相为２０：８０的乙腈：缓冲溶液（０．０１ｍｏｌＬ／Ｌ磷酸盐，０．０１％ＴＢＡ，ＰＨ７．０）检测波长为２７２ｎｍ。结果：本分析方法在（１．２

A High Performance Liquid Chromatographic Method for the Determination of Cefmetazole in Bile

Objective:To develop an analytical method for the quantitative determination of cefmetazole in bile.Method:An aliquot of 0.2 mL bile was diluted to 1 mL with deionized water and applied onto a preconditioned C18 solid phase extraction cartridge.Cefmetazole was eluted from the cartridge with 1 mL of 10% acetonitrile in water and then analyzed with ion-paired reverse phase high performance liquid chromatography with uv detection.Mobile phase was 20∶80 acetonitrile∶buffer (0.01moL/L phosphate with 0.01%TBA,pH 7.0).The wavelength of the detector was set at 272nm.Results:The analytical method gave good linearity in the concentration range of (1.25～25)μg/mL of cefmetazole in bile(γ=0.9999).The method recoveries at different concentration levels were between 88.0%～103% with within-day and day-to-day relative standard deviations less than 5.2%.The method detection limit and quantitation limit were 1.2μg/mL and 2.4μg/mL of cefmetazole in bile,respectively.