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血浆载脂蛋白A5水平与HDL亚类分布的关系
引用本文:韩瑛,陈志军,唐伟军,龙石银,卢新华,邓华菲,王岐本,田英. 血浆载脂蛋白A5水平与HDL亚类分布的关系[J]. 国际病理科学与临床杂志, 2014, 0(3): 234-237
作者姓名:韩瑛  陈志军  唐伟军  龙石银  卢新华  邓华菲  王岐本  田英
作者单位:[1]湘南学院基础医学部机能学实验室,湖南郴州423000 [2]湘南学院南岭药用资源研究所,湖南郴州423000 [3]郴州市第一人民医院病理诊断中心,湖南郴州423000 [4]南华大学生物化学与分子生物学教研室,湖南衡阳421001
基金项目:湖南省教育厅科研基金重点项目(12A127);湖南省卫生厅科研基金(B2012-74);湘南学院科研基金(2013YJ46).
摘    要:目的:探讨载脂蛋白A5(apolipoprotein A5,apoA5)水平对HDL亚类分布的影响。方法:采用酶联免疫吸附法(enzyme-1inked immunosorbent assay,ELISA)和双向电泳免疫印迹检测法分别检测310例受试者的血浆apoA5浓度和HDL亚类相对含量。受试者按apoA5浓度均值加或减去一个标准差作为分割点,分为高apoA5组(apoA5≥286.83mg/L),中apoA5组(139.99mg/L≤apoA5〈286.83mg/L)和低apoA5组(apoA5〈139.99mg/L)。结果:与高apoA5组相比,apoA5低浓度组preβl-HDL和HDL3a含量显著升高(P〈0.01),HDL2a和HDL2b显著降低(p〈0.01)。结论:随着血浆apoA5浓度降低,HDL亚类有变小的趋势,apoA5浓度降低可能阻碍了HDL亚类的成熟。

关 键 词:HDL亚类  载脂蛋白A5  双向电泳-免疫印迹检测法

Relationship between apoA5 level and HDL subclasses
HAN Ying,CHEN Zhijun,TANG Weijun,LONG Shiyin LU Xinhua,DENG Huafei,WANG Qiben,TIAN Ying. Relationship between apoA5 level and HDL subclasses[J]. Journal of International Pathology and Clinical Medicine, 2014, 0(3): 234-237
Authors:HAN Ying  CHEN Zhijun  TANG Weijun  LONG Shiyin LU Xinhua  DENG Huafei  WANG Qiben  TIAN Ying
Affiliation:1. Functional Laboratoryj Department of Basic Medicinej University of Xiangnan, Chenzhou Hunan 423000; 2. Joint Institute for Medicinal Resources Nanling, University of Xiangnan, Chenzhou Hunan 423000; 3. Pathological Diagnosis Center, First People's Hospital of Chenzhou, Chenzhou Hunan 423000; 4. Department of Biochemistry and Molecular Biology, University of South China, Hengyang Hunan 421001, China)
Abstract:Objective: To explore the relation between the apolipoprotein A5 (apoAS) levels and subclasses of high density lipoprotein (HDL). Methods: The plasma level of apoA5 was detected by enzyme-linked immunosorbent assay (ELISA), and the HDL subclasses were measured with two-dimensional electrophoresis immunoblot in 310 subjects. According to the apoA5 level, the subjects were divided into three groups: a high level of apoA5 (apoA5 ≥ 286.83 mg/L) group, a medium level ofapoA5 (139.99 ≤ apoA5 〈 286.83 mg/L) group and a lowlevel of apoA5 (apoA5 〈 139.99 mg/L) group. Results: Compared with the high level ofapoA5 group, preβl-HDL and HDL3a were significantly increased (P〈0.01), while HDL2a and HDL2b were significantly decreased (P〈O.01) in the low level of apoA5 group. Conclusion: With the decreased plasma level of apoAS, the particle size of HDL shows a shift toward smaller size, and the decreased apoA5 may suppress the mature metabolism of HDL.
Keywords:high density lipoprotein subclasses  apolipoprotein AS  two dimensional gel electrophoresis-immunodetection
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