| 桑银降糖胶囊在体外对猪胰岛细胞增殖及分泌功能的影响 |
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| 引用本文： | 任霞,唐天华,姜枫勤,任海全,温培娥,姜国胜. 桑银降糖胶囊在体外对猪胰岛细胞增殖及分泌功能的影响[J]. 山东医药, 2010, 50(43): 4-6 |
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| 作者姓名： | 任霞 唐天华 姜枫勤 任海全 温培娥 姜国胜 |
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| 作者单位： | 山东省医学科学院基础医学研究所,济南,250062 |
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| 基金项目： | 山东省科技厅科技攻关项目 |
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| 摘 要： | 目的观察桑银降糖胶囊对体外培养猪胰岛细胞分泌功能的影响。方法体外分离纯化猪胰岛细胞进行单层细胞培养,链脲霉素（STZ）处理后分别与桑银降糖胶囊及其组分桑叶多糖、牛磺酸、银杏黄酮等共同孵育24、48 h,用放射免疫法检测培养液中胰岛素、C肽水平;光镜下观察细胞形态变化;MTT法检测胰岛细胞增殖情况。结果与其他组分比较,桑银降糖胶囊可明显提高培养液中胰岛素、C肽水平,胰岛β细胞数目明显增多。结论桑银降糖胶囊可减轻STZ对胰岛β细胞的损伤,促进胰岛细胞增殖,并使损伤的胰腺恢复正常功能,增强胰岛素的分泌功能。
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| 关 键 词： | 桑银降糖胶囊 链脲霉素 胰岛细胞 细胞增殖 |
Effects of Sangyin capsule on proliferation and secretion function of porcine islet cells in vitro |
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| REN Xia,TANG Tian-hua,JIANG Feng-qin,REN Hai-quan,WEN Pei-e,JIANG Guo-sheng. Effects of Sangyin capsule on proliferation and secretion function of porcine islet cells in vitro[J]. Shandong Medical Journal, 2010, 50(43): 4-6 |
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| Authors: | REN Xia TANG Tian-hua JIANG Feng-qin REN Hai-quan WEN Pei-e JIANG Guo-sheng |
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| Affiliation: | REN Xia,TANG Tian-hua,JIANG Feng-qin,REN Hai-quan,WEN Pei-e,JIANG Guo-sheng (Institute of Basic Medicine,Shandong Academy of Medical Sciences,Jinan 250062,P.R.China) |
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| Abstract: | Objective To investigate the effects of Sangyin capsule on proliferation and secretion function of porcine islet cells in vitro.Methods Islet cells of new-born swine were separated for monolayer cell culture,after the performance of streptozotocin,the islet cells were treated with Sangyin capsule and its components including mulberry polysaccharide,taurine,and ginkgetin,respectively for 24 hs and 48 hs,morphology of islet cells in every group was observed with microscopy and the proliferation was determined... |
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| Keywords: | Sangyin capsule streptozotocin islet cells cell proliferation |
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