| 霍乱弧菌非O1群菌株的毒力基因检测和染色体酶切图谱分析 |
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| 引用本文： | 段广才,高守一,祁国明,刘延清,刘彩莲. 霍乱弧菌非O1群菌株的毒力基因检测和染色体酶切图谱分析[J]. 郑州大学学报(医学版), 1998, 0(3) |
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| 作者姓名： | 段广才 高守一 祁国明 刘延清 刘彩莲 |
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| 作者单位： | 河南医科大学公共卫生学院流行病学教研室，中国预防医学科学院流行病学微生物学研究所 |
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| 摘 要： | 非Ｏ１群霍乱弧菌（不包括Ｏ１３９菌）是腹泻病原菌，由于它们分布较广，因此对人们的健康具有较大的威协；应用核酸技术对９８株非Ｏ１菌的主要毒力基因进行检测，并从ＤＮＡ和基因水平对５株非Ｏ１菌的分子特征进行研究。结果：所选菌株的染色体酶切图谱与Ｏ１群霍乱弧菌相比有很大差异，尤其与Ｏ１群流行株的差异更大，非Ｏ１菌相互之间的差异也很大；对９８株非Ｏ１菌进行ｃｔｘ、ｚｏｔ、ａｃｅ、ＲＳ１基因检测，结果有２株为上述基因阳性，仅为２％左右。结果显示：非Ｏ１菌群体遗传结构复杂，变异程度高；其与Ｏ１菌，尤其是流行株（包括Ｏ１３９菌）的遗传关系较远；结果也说明大多数非Ｏ１菌是不致病的
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| 关 键 词： | 非O1群霍乱弧菌；毒力基因；脉冲电泳；分子杂交；分子特征 |
DNA restriction patterns and the distribution of virulence genes of Vibrio cholerae non O1 |
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| Duan Guangcai ),Gao Shouyi ),Qi Guoming ),Liu Yanqing ),Liu Cailian ) ). DNA restriction patterns and the distribution of virulence genes of Vibrio cholerae non O1[J]. Journal of Zhengzhou University: Med Sci, 1998, 0(3) |
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| Authors: | Duan Guangcai ) Gao Shouyi ) Qi Guoming ) Liu Yanqing ) Liu Cailian ) ) |
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| Affiliation: | Duan Guangcai 1),Gao Shouyi 2),Qi Guoming 2),Liu Yanqing 2),Liu Cailian 2) 1)Department of Epidemiology,Henan Medical University,Zhengzhou 450052 2)Institute of Epidemiology and Microbiology,Chinese Academy of Preventive |
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| Abstract: | Vibrio cholerae (VC) non O1 (excluding VC O139) can be isolated from the environmental water almost through out the world. Part of them are able to cause human diarrhea. Furthermore some of the researchers considered that VC non O1 are the sources of the new type of Vibrio cholerae. So VC non O1 are the potential pathogens of the humans. In this paper 98 isolates of VC non O1 were detected for ctx, zot, ace and RS1 with colony hybridization and 5 isolates of them were studied with pulsed field gel electrophoresis. The results showed that there were significant differences between the chromosomal DNA restriction patterns of VC non O1 and VC O1, especially the epidemigenic strains of VC O1; By the colony hybridization with the gene probes of ctx, zot, ace and RS1, only two of ninety eight isolates of VC non O1 were positive. These results confirmed that only a very little part of VC non O1 which possess the identical molecular characteristics and virulence genes are pathogenic to humans. |
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| Keywords: | Vibrio cholerae non O1 virulence genes pulsed field gel electrophoresis molecular hybridization molecular characteristics |
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