香附超临界C02萃取物化学成分分析

目的:提取香附的有效部位并研究其不同有效部位的化学成分。方法:采用超临界CO2萃取技术从香附药材中提取香附超临界CO2萃取物(香附-SFE),再用分子蒸馏技术对香附-SFE作进一步处理,从而富集有效成分。并使用气质联用技术对香附超临界CO2萃取物及分子蒸馏处理所得到有效部位的化学成分分别进行了分析。结果:香附-SFE分子蒸馏前后挥发油的含量由43.2%上升到86.0%,香附子烯和α-香附酮的相对含量由20.2%提高到38.6%。香附-MD1中未检测到脂肪酸类组分。结论:分子蒸馏技术可对香附-SFE进一步精制,有效保留挥发油和α-香附酮等有效成分,去除脂肪酸类等无效成分。

Study on the Chemical Substrates of SFE Extract from Rhizoma Cyperi

OBJECTIVE: To extract the effective part of Rhizoma Cyperi and study the chemical constituents of diffrent parts. METHODS: The extract of Rhizoma Cyperi was obtained by SFE and dealed with molecular distillation, then the effective components was enriched. The chemical compositions were separated and identified by GC/MS. RESULTS: The content of volatile oil in the extract upgraded from 43.2% to 86.0% by molecular distillation, and the relative amount of cyperene and (+) -alpha-cyperone from 20.2% to 38.6%. Fatty acids was not found in Rhizoma Cyperi-MD1. CONCLUSION: The SFE of Rhizoma Cyperi could be refined with molecular distillation. The active ingredients, such as volatile oil and alpha-cyperolone, were remained effectively. The ineffective ingredients fatty acids were removed.