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Good performance of p16/ki‐67 dual‐stained cytology for surveillance of women treated for high‐grade CIN
Authors:Nicole J Polman  Margot H Uijterwaal  Birgit I Witte  Johannes Berkhof  Folkert J van Kemenade  Johan WM Spruijt  W Marchien van Baal  Peppino GCM Graziosi  Dorenda KE van Dijken  René HM Verheijen  Theo JM Helmerhorst  Renske DM Steenbergen  Daniëlle AM Heideman  Ruediger Ridder  Peter JF Snijders  Chris JLM Meijer
Institution:1. Department of Pathology, VU University Medical Center, Amsterdam, The Netherlands;2. Department of Epidemiology and Biostatistics, VU University Medical Center, Amsterdam, The Netherlands;3. Department of Pathology, Erasmus MC University Medical Center, Rotterdam, The Netherlands;4. Department of Obstetrics and Gynecology, VU University Medical Center, Amsterdam, The Netherlands;5. Department of Obstetrics and Gynecology, Flevo Hospital, Almere, The Netherlands;6. Department of Obstetrics and Gynecology, Sint Antonius Hospital, Nieuwegein, The Netherlands;7. Department of Obstetrics and Gynecology, Onze Lieve Vrouwen Gasthuis West, Amsterdam, The Netherlands;8. Department of Gynaecological Oncology, Division of Surgical Oncology, UMC Utrecht Cancer Center, Utrecht, The Netherlands;9. Department of Obstetrics and Gynecology, Erasmus MC University Medical Center, Rotterdam, The Netherlands;10. Roche mtm Laboratories AG, Mannheim, Germany;11. Ventana Medical Systems, Inc., Tucson, Arizona
Abstract:Women treated for high‐grade cervical intraepithelial neoplasia (CIN) are at risk of recurrent CIN Grade 2 or worse (rCIN2+). Currently, posttreatment monitoring is performed using cytology or cytology/high‐risk (hr)HPV cotesting. This study aimed to evaluate the performance of p16/Ki‐67 dual‐stained cytology (p16/Ki‐67) for posttreatment monitoring. Three hundred and twenty‐three women treated for high‐grade CIN in the SIMONATH study underwent close surveillance by cytology, hrHPV and DNA methylation marker testing up to 12 months posttreatment. Histological endpoints were ascertained by colposcopy with biopsy at 6 and/or 12 months. p16/Ki‐67 dual‐staining was performed on residual liquid‐based cytology samples obtained at, or shortly before biopsy collection. Clinical performance estimates of cytology, hrHPV, p16/Ki‐67 testing and combinations thereof for the detection of rCIN2+ were determined and compared to each other. Sensitivity of p16/Ki‐67 for rCIN2+ (69.2%) was nonsignificantly lower than that of cytology (82.1%; ratio 0.84, 95% CI: 0.71–1.01), but significantly lower than that of hrHPV testing (84.6%; ratio 0.82, 95% CI: 0.68–0.99). Specificity of p16/Ki‐67 for rCIN2+ (90.4%) was significantly higher compared to both cytology (70.8%; ratio 1.28, 95% CI: 1.19–1.37) and hrHPV testing (76.2%; ratio 1.19, 95% CI: 1.12–1.26). Overall, hrHPV testing showed very high sensitivity, along with a good specificity. When considering cotesting, combined p16/Ki‐67/hrHPV testing showed rCIN2+ sensitivity comparable to cytology/hrHPV cotesting (87.2% vs. 89.7%; ratio 0.97, 95% CI: 0.92–1.03), but with significantly increased specificity (74.2% vs. 58.1%; ratio 1.28, 95% CI: 1.19–1.38). Thus, when considered in combination with hrHPV, p16/Ki‐67 might be an attractive approach for surveillance of women treated for high‐grade CIN.
Keywords:p16/Ki‐67 dual‐stained cytology  cervical cytology  human papillomavirus  recurrent cervical intraepithelial neoplasia  posttreatment
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