弓形虫速殖子在宿主细胞内增殖与丝裂原激活蛋白激酶表达

目的:观察RH株弓形虫速殖子在不同培养基中及在NIH3T3和MRC5细胞内的增殖,比较其丝裂原激活蛋白激酶(MAPK)表达的差异.方法:速殖子细胞培养观察宿主细胞的感染率,不同培养时间观察培养基中游离速殖子数量,Western blot分析在不同条件下MAPK的表达.结果:在MEM培养基中RH株速殖子的增殖速度明显高于在DMEM培养基中的增殖速度;其感染细胞百分率和培养基中游离速殖子计数均有显著差别;Western blot分析表明在二者具有MAPK识别的差异.结论:MEM和DMEM两种培养基对RH株速殖子的增殖有显著影响,初步结果表明其差异与MAPK有关.

Proliferation of Toxoplasma gondii tachyzoites and its expression of mitogen activated protein kinases in host cells

Objective:To observe the proliferation of RH strain tachyzoites of Toxoplasma gondii in media MEM and DMEM and in different host cell lines,and to compare the difference in expression of mitogen activated protein kinases(MAPK).Methods:Cell culture and Western blot were used for detecting the infective rate and revealing the difference in MAPK expression.Results:The tachyzoites proliferated more quickly in medium MEM than in medium DMEM,and the quantity of free tachyzoites differed remarkably in these two media.In Western blot analysis,a special MAPK expression of p80 was shown in DMEM culture system.Conclusions:Some factors in the media influence the proliferation of tachyzoites,which relates MAPK expression.