三氧化二砷联合热疗对人食管癌细胞株EC-1增值和细胞周期的影响

摘要：目的 探讨三氧化二砷(AS203) 联合热疗对人食管癌细胞株EC-1增殖的抑制作用及机制。方法 经AS203注射液联合热疗处理EC-1细胞后采用MTT法测定细胞的增殖抑制效应; 应用流式细胞术检测细胞周期变化，TUNEL法观察细胞凋亡。结果:与AS203单药或单用热疗相比, AS203与热疗联合应用后可显著抑制EC-1细胞增殖和诱导细胞凋亡, 并使细胞S期和G0/G1期比例明显降低, G2 /M 期明显升高。结论:AS203联合热疗具有协同抗食管癌作用,其机制可能与联合应用后增强诱导食管癌细胞凋亡、细胞周期特异性治疗与细胞周期调控剂联合增效有关。

The Effect of Arsenic Trioxide(AS203) in Conjunction with Hyper- thermia on Proliferation and Cell Cycle in human esophageal carcinoma EC-1cell line

[Abstract] Objective: To study the effect of arsenic trioxide(AS203) combined with hyperthermia on the growth of esophageal carcinoma EC-1 cell line and to discuss its possible mechanism. Methods: In every group ,the percentage of survival was calculated with MTT assay, the apoptosis and the cell cycle were observed by flow cytometry and TUNEL. Results: AS203 combined with hyperthermia could obviously inhibit cell proliferation and induce apoptosis as compared with AS203 or hyperthermia.The Flow cytometry assay showed that AS203 combined with hyperthermia could induced G2/M phase arrest and the reduction of the ratio of cells in G0/G1 and S phases expectively. Conclusion: AS203 in combination with hyperthermia can produce synergistic killing effect on EC-1 cell. This may be related with the increase of apoptosis and cell cycle specific killing combined with cell cycle regulator.