| 脱病毒半夏中半夏蛋白的含量测定 |
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| 引用本文： | 田晖,冯瑞娟,章伟标,徐涛. 脱病毒半夏中半夏蛋白的含量测定[J]. 时珍国医国药, 2011, 22(9): 2064-2065. DOI: 10.3969/j.issn.1008-0805.2011.09.002 |
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| 作者姓名： | 田晖 冯瑞娟 章伟标 徐涛 |
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| 作者单位： | 1. 杭州职业技术学院,浙江,杭州,310018
2. 浙江理工大学生命科学学院,浙江,杭州,310018 |
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| 基金项目： | 国家自然基金面上项目，浙江省杭州市科技局项目 |
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| 摘 要： | 目的测定脱病毒半夏块茎及种植半夏中总蛋白的含量。方法采用Folin-酚法测定半夏块茎中总蛋白含量。结果于波长750 nm处比色,标准曲线线性良好,线性方程为Y=0.001 47 X+0.011 08r,=0.999 4,脱病毒半夏蛋白含量为2.86%。结论 Folin-酚法简便、快速、灵敏度高,可以用于半夏块茎中总蛋白含量的测定。
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| 关 键 词： | Folin-酚法 脱病毒半夏 蛋白含量 |
Determination of Total Protein Content in Virus Eliminated Pinellia tuber by Folin-phenol |
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| TIAN Hui,FENG Rui-juan,ZHANG Wei-biao,XU Tao. Determination of Total Protein Content in Virus Eliminated Pinellia tuber by Folin-phenol[J]. Lishizhen Medicine and Materia Medica Research, 2011, 22(9): 2064-2065. DOI: 10.3969/j.issn.1008-0805.2011.09.002 |
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| Authors: | TIAN Hui FENG Rui-juan ZHANG Wei-biao XU Tao |
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| Affiliation: | TIAN Hui1,FENG Rui-juan2,ZHANG Wei-biao2,XU Tao2(1.Hangzhou Vocational and Technical College,Hangzhou 310018,2.College of Life Science,Zhejiang University of Sci-Tech,China) |
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| Abstract: | Objective To determine the total protein content in virus eliminated Pinellia tuber. Methods The total protein content in Pinellia tuber was determined by the Folin-phenol method. Results The standard curve was good,the linear equation was Y=0.001 47x+0.011 08,R=0.999 4,the wavelength was 750 nm,and the total protein content of the samples was 2.86%. Conclusion Folin-phenol method is simple and fast,and it can be used to determine the total protein content in Pinellia tuber. |
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| Keywords: | Folin-phenol method Virus eliminated pinellia Total protein content |
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