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| 狼毒对荷瘤小鼠体内恶性黑色素瘤细胞的抑制作用及其分子机制研究 | |
| 引用本文： | 王莉平,王义善,张国营,刘昆,段慧英,马念. 狼毒对荷瘤小鼠体内恶性黑色素瘤细胞的抑制作用及其分子机制研究[J]. 时珍国医国药, 2011, 22(11). DOI: 10.3969/j.issn.1008-0805.2011.11.047 |
| 作者姓名： | 王莉平 王义善 张国营 刘昆 段慧英 马念 |
| 作者单位： | 1. 滨州医学院附属医院,山东滨州256603;滨州医学院中西医结合学院,山东烟台264003 2. 滨州医学院中西医结合学院,山东烟台,264003 3. 烟台大学药学院,山东烟台,264003 4. 滨州医学院附属医院,山东滨州,256603 |
| 基金项目： | 中国人民解放军全军科技攻关计划基金资助项目 |
| 摘 要： | 目的 观察狼毒大戟提取液(LDE)对荷瘤小鼠恶性黑色素瘤B16细胞增殖的影响,并探讨其可能的作用机制.方法 观察荷瘤小鼠空白组和狼毒治疗组的肿瘤生长情况；采用ELISA法检测小鼠血清中血管内皮生长因子(VEGF)、肝细胞生长因子(HGF)和骨桥蛋白(OPN)的活性；Western Blot蛋白印迹法检测了狼毒大戟提取液对荷瘤小鼠黑色素瘤B16组织中的Bcl - 2/Bax凋亡蛋白的表达.结果 与对照组相比较,狼毒大戟提取液对荷瘤小鼠体内恶性黑色素瘤B16细胞的生长有显著的抑制作用,能明显减慢肿瘤体积生长趋势和减轻瘤重；与对照组相比较,狼毒治疗组的VEGF、OPN的水平有统计学意义(P＜0.05)；HGF的的水平有显著的统计学意义(P＜0.01)；狼毒能下调小鼠体内恶性黑色素瘤B16中Bcl - 2/Bax凋亡蛋白的表达.结论 狼毒大戟提取液是一种有效的抗恶性黑色素瘤B16细胞增殖的中药水提液成分.其作用机制可能与诱导细胞凋亡,影响细胞中相关蛋白表达以及肿瘤血管生长和肿瘤转移相关因子VEGF、HGF、OPN的活性有关. |
| 关 键 词： | 狼毒大戟 恶性黑色素瘤B16 增殖 抑制作用 VEGF HGF OPN 细胞凋亡 |
Effects of LDE on the Growth and Metastasis of Malignant B16 Melanoma and its Mechanism |
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| WANG Li-ping,WANG Yi-shan,ZHANG Guo-ying,LIU Kun,DUAN Hui-ying,MA Nian. Effects of LDE on the Growth and Metastasis of Malignant B16 Melanoma and its Mechanism[J]. Lishizhen Medicine and Materia Medica Research, 2011, 22(11). DOI: 10.3969/j.issn.1008-0805.2011.11.047 | |
| Authors: | WANG Li-ping WANG Yi-shan ZHANG Guo-ying LIU Kun DUAN Hui-ying MA Nian |
| Affiliation: | WANG Li-ping 1,3,WANG Yi-shan 3*,ZHANG Guo-ying2,LIU Kun2,DUAN Hui-ying2,MA Nian1 (1.Affiliated Hospital of Binzhou Medical College,Binzhou256603,China,2.Yantai University,Yantai264005,3.Binzhou Medical College,Yantai264003,China) |
| Abstract: | Objective To study the effects of LDE on the proliferation of malignant B16 melanoma cells and its mechanisms. Methods The serum levels of VEGF,HGF and OPN were measured by ELISA.Western blot was used to detect the expression of Bcl-2/Bax apoptosis proteins in malignant melanoma B16 cells of tumor-bearing mice. Results Compared with the control group,LDE had effect on the volume and weight of tumors in melanoma-bearing mice.LDE could inhibit the growth of malignant B16 melanoma cells in mice,reduce serum le... |
| Keywords: | Euphorbia fischeriana Steud Malignant B16 melanoma Proliferation Inhibition VEGF HGF OPN Apoptosis |
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