| 血府逐瘀汤对大鼠血管平滑肌细胞MMP-2、TIMP-1表达的影响 |
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| 引用本文： | 林薇,赵锦燕,郑良朴,曹治云,陈旭征,彭军. 血府逐瘀汤对大鼠血管平滑肌细胞MMP-2、TIMP-1表达的影响[J]. 时珍国医国药, 2011, 22(11). DOI: 10.3969/j.issn.1008-0805.2011.11.042 |
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| 作者姓名： | 林薇 赵锦燕 郑良朴 曹治云 陈旭征 彭军 |
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| 作者单位： | 1. 福建中医学院福建省高校中西医结合基础重点实验室,福建福州350108;福建中西医结合研究院,福建福州350108
2. 福建中西医结合研究院,福建福州,350108 |
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| 基金项目： | 福建省自然科学基金，福建省中西医结合老年性疾病重点实验室开放课题，福建省教育厅课题 |
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| 摘 要： | 目的 观察血府逐瘀汤含药血清对大鼠血管平滑肌细胞(VSMCs)迁移、侵袭的影响,以及其生成MMP -2、TIMP -1情况,探讨其作用机制.方法 通过划痕损伤实验、Boyden - chamber细胞侵袭实验观察VSMCs的迁移、侵袭,RT - PCR检测血府逐瘀汤含药血清对基质金属蛋白酶-2(MMP -2)和基质金属蛋白酶抑制剂-1(TIMP-1)的mRNA表达.结果 与空白对照组比较,血府逐瘀汤高剂量组和中剂量组能显著抑制VSMCs的增殖和迁移(P＜0.05)；与空白对照组比较,血府逐瘀汤组均能明显下调MMP-2 mRNA的表达同时上调TIMP-1 mRNA的表达(P＜0.01).结论 血府逐瘀汤含药血清有抑制VASMCs迁移和侵袭的作用,MMP -2表达降低、TIMP -1的表达升高可能是其机制之一.
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| 关 键 词： | 血府逐瘀汤 血管平滑肌细胞 基质金属蛋白酶-2 金属蛋白酶抑制剂-1 |
Effect of XueFuZhuYu Decoction on MMP - 2 and TIMP - 1 Expression of Vascular Smooth Muscle Cells from Rats |
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| LIN Wei,ZHAO Jin-yan,ZHENG Liang-pu,CAO Zhi-yun,CHEN Xu-zheng,PENG Jun. Effect of XueFuZhuYu Decoction on MMP - 2 and TIMP - 1 Expression of Vascular Smooth Muscle Cells from Rats[J]. Lishizhen Medicine and Materia Medica Research, 2011, 22(11). DOI: 10.3969/j.issn.1008-0805.2011.11.042 |
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| Authors: | LIN Wei ZHAO Jin-yan ZHENG Liang-pu CAO Zhi-yun CHEN Xu-zheng PENG Jun |
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| Affiliation: | LIN Wei 1,2,ZHAO Jin-yan 1,ZHENG Liang-pu 1,CAO Zhi-yun2,CHEN Xu-zheng2,PENG Jun 1,2* (1.Fujian Academy of Integrative Medicine,Fujian University of Traditional Chinese Medicine,Fuzhou,350108,China,2.Fujian Academy of Integrated Traditional Chinese Medicine and Western Medicine,Fuzhou 350108,China) |
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| Abstract: | Objective To observe the effect of XueFuZhuYu Decoction on migration and invasion of vascular smooth muscle cells from rats and its mechanism. Methods Cell migration was determine by wound healing assay,and invasion was determined by transwell assay.mRNA expression of MMP-2 and TIMP-1 was detected by RT-PCR analysis. Results Compared with blank group,both high and middle dose groups could inhibit migration and invasion,and mRNA expression of MMP-2 was down-regulated,meanwhile mRNA expression of TIMP-1 was u... |
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| Keywords: | XueFuZhuYu Decoction Vascular smooth muscle cell Matrix metalloproteinase-2 Tissue inhibitor of matrix metalloproteinase-1 |
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