| 复叶耳蕨提取物体外抗HIV-1 RT活性的研究 |
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| 引用本文： | 李辉敏,殷嫦嫦,吴家忠,张磊.复叶耳蕨提取物体外抗HIV-1 RT活性的研究[J].时珍国医国药,2011,22(9):2081-2082. |
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| 作者姓名： | 李辉敏 殷嫦嫦 吴家忠 张磊 |
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| 作者单位： | 九江学院·基础医学院,江西,九江,332000 |
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| 摘 要： | 目的对鳞毛蕨科复叶耳蕨属植物复叶耳蕨不同提取部位体外抗HIV-1逆转录酶活性进行研究。方法在体外无细胞系统内采用酶联免疫吸附测定HIV-1逆转录酶的半数有效浓度(IC50)。结果复叶耳蕨乙酸乙酯提取物、正丁醇提取物抑制HIV-1逆转录酶的半数有效浓度IC50分别为84.069,.74μg.ml-1,水提取物抑制HIV-1逆转录酶的半数有效浓度IC50>200μg.ml-1。结论复叶耳蕨乙酸乙酯提取物与正丁醇提取物具有体外抑制HIV-1逆转录酶作用。
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| 关 键 词： | 复叶耳蕨 不同提取部位 HIV-1逆转录酶 |
Anti-HIV-1 RT Effect of Different Extracts from Arachniodes exilis Ching |
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| LI Hui-min,YIN Chang-chang,WU Jia-zhong,ZHANG Lei.Anti-HIV-1 RT Effect of Different Extracts from Arachniodes exilis Ching[J].Lishizhen Medicine and Materia Medica Research,2011,22(9):2081-2082. |
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| Authors: | LI Hui-min YIN Chang-chang WU Jia-zhong ZHANG Lei |
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| Institution: | LI Hui-min,YIN Chang-chang,WU Jia-zhong,ZHANG Lei (Medical School,Jiujiang College,Jiangxi,Jiujiang 332000,China) |
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| Abstract: | Objective To evaluate the anti-HIV-1 RT activities of the different extracts from Arachniodes exilis Ching. Methods The inhibitory effect on HIV-1 RT was detected by enzyme-linked immunosorbent assay. Results In the experiment of anti-HIV RT,the IC50 of the n-butanol extract、acetic ether extract were 84.06,9.74μg·ml-1,respectively.The IC50 of the aqueous extract was higher than 200 μg·ml-1. Conclusion The n-butanol extract,acetic ether extract from Arachniodes exilis Ching have the inhibitory effect on HIV-1 RT. |
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| Keywords: | Arachniodes exilis Different extracted parts HIV-1 RT |
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