中焦湿阻证Cajal细胞内乳酸脱氢酶和琥珀酸脱氢酶活力变化及平胃散的干预研究

目的探索中焦湿阻证模型ICC细胞和原代ICC细胞的差异,建立湿阻ICC细胞模型,和经平胃散干预后细胞内乳酸脱氢酶(LDH)和琥珀酸脱氢酶(SDH)活力的变化反证模型细胞和原代ICC存在差异。方法湿阻中焦动物模型建立及动物血清制备;采用Ⅱ胶原酶消化法体外培养ICC,建立"中焦湿阻证Cajal间质细胞模型",给细胞加入湿阻动物含药血清模拟湿阻大鼠模型体内ICC的生长;通过给ICC细胞加入动物血清,观察平胃散对ICC细胞内LDH和SDH活力的影响。结果中焦湿阻证ICC细胞模型的评价:模型细胞与原代细胞有差异。细胞内LDH活力结果提示,与原代ICC+模型血清组及原代ICC+2次模型血清组比较,平胃散含药血清能降低加入1次血清组及加入2次血清组细胞内LDH活力(P<0.01)。细胞内SDH活力结果提示:平胃散含药血清能升高加入1次血清组细胞内SDH活力(P<0.01),而对加入2次血清组细胞内SDH活力有升高趋势。结论平胃散能降低中焦湿阻证Cajal间质细胞模型细胞内LDH活力和升高SDH活力,抑制细胞内无氧代谢增强细胞内有氧代谢。

Changes of LDH and SDH Vitality in Damp Blockage of Middle Energizer ICC Cells Model and the Intervention of PING WEI SAN

Objective To explore the difference between middle energizer of damp blockage ICC cells and original generation ICC cells. Methods Establish damp blockage of middle energizer model in rats and prepare rats’ model serum.Collagenase Ⅱ was used to digests external cultivated ICC,adding PING WEI SAN serum of damp blockage rats to the cells after successful identification,which simulate the growth damp blockage rats’ ICC cells.Set up "the model of damp blockage of middle energizer in ICC cells" and elevate the model.The effect of PING WEI SAN on the vitality of LDH and SDH in ICC cells was observed. Results Model cells had discrepancies with normal ICC.Compared with model groups,PING WEI SAN in serum could reduce the vitality of LDH in normal ICC cells and the model ICC(P <0.01),elevate the vitality of SDH in ICC cells(P <0.01). Conclusion PING WEI SAN can reduce the vitality of LDH in model ICC cells and increase the vitality of SDH in model ICC cells,suppress internal cell anaerobic metabolism and increase internal cell aerobic metabolism.