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| 苦丁茶熊果酸对鼻咽癌细胞增殖的抑制作用研究 | |
| 引用本文： | 农朝赞,李世生,毛德文,黄之虎,郭凌霄,韦仕喻,农少云.苦丁茶熊果酸对鼻咽癌细胞增殖的抑制作用研究[J].时珍国医国药,2011,22(11). |
| 作者姓名： | 农朝赞 李世生 毛德文 黄之虎 郭凌霄 韦仕喻 农少云 |
| 作者单位： | 1. 广西壮族自治区民族医院,广西南宁,530001 2. 广西医科大学第一附属医院,广西南宁,530021 3. 广西中医学院第一附属医院肝病中心,广西南宁,530021 |
| 基金项目： | 广西壮族自治区青年自然科学基金 |
| 摘 要： | 目的 探讨苦丁茶熊果酸对鼻咽癌细胞的抑制作用,为苦丁茶的深加工提供研究依据.方法 从苦丁茶老叶中提取熊果酸并纯化与鉴定,用5,10,20,40,80,100μmol·L-6个梯度浓度的熊果酸,持续作用于在培养的鼻咽癌细胞24,48,72,96,120 h后,用四甲基偶氮唑蓝比色法检测熊果酸对鼻咽癌细胞的抑制作用情况,流式细胞术分析其细胞周期和凋亡率.结果 不同浓度的苦丁茶熊果酸在不同时间内对鼻咽癌细胞均具有较强的抑制作用,并随浓度的升高及作用时间的延长而作用增强；流式细胞术结果显示,苦丁茶熊果酸阻滞鼻咽癌细胞于G0/G1期；40 μmol/L熊果酸作用48 h后抑制效果逐渐明显.结论 从苦丁茶提取的熊果酸对鼻咽癌细胞有较强的抑制作用,能阻滞癌细胞于G0/G1期,并诱导肿瘤细胞凋亡,苦丁茶极具开发前景. |
| 关 键 词： | 营丁茶 熊果酸 鼻咽癌细胞 细胞凋亡 |
The Proliferation Inhibition Effect of Ursolic Acid from Kuding Tea on NCE Carcinoma Cell |
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| NONG Chao-zan,LI Shi-sheng,MAO De-wen,HUANG Zhi-hu,GUO Ling-xiao,WEI Shi-yu,NONG Shao-yun.The Proliferation Inhibition Effect of Ursolic Acid from Kuding Tea on NCE Carcinoma Cell[J].Lishizhen Medicine and Materia Medica Research,2011,22(11). | |
| Authors: | NONG Chao-zan LI Shi-sheng MAO De-wen HUANG Zhi-hu GUO Ling-xiao WEI Shi-yu NONG Shao-yun |
| Institution: | NONG Chao-zan1,LI Shi-sheng2,MAO De-wen3,HUANG Zhi-hu1,GUO Ling-xiao1,WEI Shi-yu1,NONG Shao-yun1 (1.Department of Experimental Center,Guangxi Nationalities Hospital,Guangxi Nanning 530001,China,2.The First Hospital of Guangxi Medical University,Nanning 530021,3.The First Hospital Affiliated to Guangxi Traditional Chinese Medical College,Nanning 530023,Guangxi,China) |
| Abstract: | Objective To observe the toxicity effect,apoptosis induction and cell cycle inhibition of ursolic acid on human nasopharyngeal carcinoma cell,and to explore the posibility of Kuding tea as a new tumor chemopreventive drug. Methods MTT method was used to determine the effect of ursolic acid on cell proliferation inhibition,microscopic method was used to observe cytotoxicity of ursolic acid,flow cytometer method was used to determine apoptosis induction and cell cycle blocking effect of ursolic acid. Results ... |
| Keywords: | Kuding tea Ursolic acid Nasopharyngeal carcinoma cell Apoptosis |
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