| 川芎嗪对肾脏缺血/再灌注大鼠GRP78和Caspase-12表达的影响 |
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| 引用本文： | 万慧芳,王美凤,涂硕,余乐涵,龚慧,万福生. 川芎嗪对肾脏缺血/再灌注大鼠GRP78和Caspase-12表达的影响[J]. 时珍国医国药, 2011, 22(11): 2620-2622. DOI: 10.3969/j.issn.1008-0805.2011.11.021 |
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| 作者姓名： | 万慧芳 王美凤 涂硕 余乐涵 龚慧 万福生 |
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| 作者单位： | 1. 南昌大学医学院,江西南昌,330006
2. 江西省南昌市第一医院330008 |
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| 基金项目： | 江西省自然科学基金，江西省科技支撑计划重点项目 |
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| 摘 要： | 目的观察川芎嗪(TMP)对肾缺血/再灌注(I/R)大鼠肾脏GRP78、Caspase-12表达的影响及其意义。方法将Wistar大鼠30只随机分为假手术组(对照组)、I/R组和I/R+TMP组。采用夹闭双侧肾蒂45min再灌注24h制备肾I/R模型。I/R+TMP组在手术前1h按20mg/kg腹腔注射TMP,余操作同I/R组。检测血清尿素氮(BUN)和肌酐(Cr);光镜观察肾小管组织结构变化;RT-PCR和免疫组化检测GRP78、Caspase-12 mRNA和蛋白表达。结果与假手术组比较,I/R组大鼠血清BUN,Cr水平显著升高,肾组织损伤严重,GRP78和Caspase-12 mRNA和蛋白表达呈显著性增加(P<0.01);与I/R组比较,I/R+TMP组血清BUN、Cr水平及GRP78、Caspase-12表达均有显著性下降(P<0.01),肾脏损伤明显减轻。结论川芎嗪对肾脏缺血/再灌注大鼠GRP78、Caspase-12过度表达有很好的抑制作用,这可能是它减轻肾脏缺血/再灌注损伤的重要机制之一。
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| 关 键 词： | 缺血再灌注损伤 川芎嗪 葡萄糖调节蛋白78 胱冬肽酶 肾脏 |
Effect of Tetramethylpyraxine on Expression of Genes GRP78 and Caspase - 12 of the Renal Tissue at Rats in Ischemia Reperfusion Injury |
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| WAN Hui-fang,WANG Mei-feng,TU Shuo,YU Le-han,GONG Hui,WAN Fu-sheng. Effect of Tetramethylpyraxine on Expression of Genes GRP78 and Caspase - 12 of the Renal Tissue at Rats in Ischemia Reperfusion Injury[J]. Lishizhen Medicine and Materia Medica Research, 2011, 22(11): 2620-2622. DOI: 10.3969/j.issn.1008-0805.2011.11.021 |
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| Authors: | WAN Hui-fang WANG Mei-feng TU Shuo YU Le-han GONG Hui WAN Fu-sheng |
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| Affiliation: | WAN Hui-fang1,WANG Mei-feng2,TU Shuo1,YU Le-han1,GONG Hui1,WAN Fu-sheng1 (1.Medical College of Nanchang University,Nanchang 330006,China,2.The First Hospital of Nanchang,Nanchang 330008,China) |
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| Abstract: | Objective To observe the effects of tetramethylpyraxine(TMP) on expressions of GRP78,Caspase-12 of the renal tissue at rats in renal ischemia /reperfusion(I/R) injury. Methods Thirty Wistar rats of either sex were randomly divided into sham operation group(control group),I/R group and I/R+TMP group.Prepare the animal model of acute renal I/R injury through clamping bilateral renal pedicle 45min and then reperfusion 24h.I/R+TMP group: intraperitoneal injection of 20mg/kg TMP solution 1h before surgery and th... |
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| Keywords: | Ischemia reperfusion injury Tetramethylpyraxine Glucose regulating protein Caspase Kidney |
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