鲍曼不动杆菌耐药基因aac（6＇）-Ib和aac（6＇）-Ib-cr的检测

目的了解阿米卡星耐药的鲍曼不动杆菌中aac（6＇）-Ib-cr基因的分布情况。方法收集2007年1月2009年10月临床标本中分离对阿米卡星耐药的鲍曼不动杆菌73株，利用聚合酶链反应（PCR）方法扩增aac（6＇）-Ib基因，PCR产物进行电泳分析和测序，aac（6＇）-Ib基因阳性PCR产物用内切酶FOK酶切后电泳分析。统计菌株对亚胺培南等12种抗生素的耐药率。结果aac（6＇）-Ib基因的检出率为89．0％，其中7．7％为突变了的aac（6＇）-Ib-cr基因。所有菌株对除亚胺培南外的其它抗生素的耐药率均大于80％。结论aac（6’）．Ib基因广泛存在于这些耐药菌株中，突变成aac（6＇）-Ib-cr基因的发生率较低。这些对阿米卡星耐药的菌株，对其它抗生素的耐药也十分严重。

Detection of aac（6＇）-Ib and aac（6＇）-Ib-cr gene in Acinetobacter baumannii

Objective To investigate the distribution of aac（6＇）-Ib-cr gene in acinetobacter baumannii resistant to amikacin. Methods A total of 73 amikacin-resistant acinetobacter banmannii from Jan 2007 to Oct.2009 were collected, aac（6＇）-Ib gene, were detected by PCR-based assays. The PCR products were electrophoretic analyzed and sequenced. PCR products of aac（6＇）-Ib gene were digested with FOK I and electrophoretic analyzed. Resistant rates to 12 antibiotics were calculated. Results The detection rates of aac（6＇）-Ib and aac（6＇）-Ib-cr gene were 89.0%, 7.7%, in 73 acinetobacter baumannii, respectively. The resistant rates of 73 strains to other antibiotics were more than 80% except imipenem. Conclusions aac（6＇）-Ib gene existed widely in clinical isolates of acinetobacter baumannii. These amikacin-resistant acinetobacter baumannii also confer high-level resistance to other antibiotics.