IL-11对ITP患者Th1、Th2细胞及T-bet、GATA-3 mRNA的影响

目的：研究白细胞介素-11 （IL-11）对免疫性血小板减少症（ITP）患者Th1、Th2细胞亚群及其调控因子T-bet mRNA和GATA-3 mRNA表达的影响.方法：从10例ITP患者静脉中抽取外周血提取单个核细胞,加入不同浓度IL-11（0、25、50、100、200 ng/mL）培养4d后,应用流式细胞术和实时荧光定量反转录-多聚酶链反应法检测Th1、Th2细胞亚群比例及T-bet mRNA、GATA-3 mRNA表达水平.结果：当IL-11浓度为100 ng/mL时,与浓度为0的对照组差异最明显,表现为Th1比例及Th1/Th2比值下降,Th2比例升高,差异有统计学意义（P＜0.01）,同时T-bet mRNA表达下降,GATA-3 mRNA表达增加,T-bet/GATA-3比值显著下降,差异有统计学意义（P＜0.01）.结论：一定浓度的IL-11在体外培养中能促使ITP患者Th1细胞向Th2细胞转化,同时GATA-3 mRNA表达增加、T-bet mRNA表达下降.

Effect of IL-11 on Th1, Th2 cells and T-bet,GATA-3 mRNA in patients with ITP

Objective： To study the effect of IL-11 on Thl, Th2 cells and the expression of modulatory gene T-bet mRNA and GATA-3 mRNA in patients with ITP. Methods： Extracting PBMNC from peripheral blood of ten patients with ITP and using different concentration of IL-11 （0, 25, 50, 100, 200 ng/mL） to cultivate PBMNC for 4 days. Detecting the proportion ofThl, Th2 cells and the quantity ofT-bet mRNA, GATA-3 mRNA by flow cytometry and quamtiy RT-PCR. Results： In different concentrations of IL-11, the differences of Thl, Th2 and the ratio of Thl/Th2 were the most significant between 0 ng/mL group and 100 ng/mL group, it showed that the level of Thl and the ratio of Thl/Th2 decreased, the level of Th2 increased in the 100 ng/mL group. The differences of T-bet mRNA, GATA-3 mRNA, and the ratio of T-bet/GATA-3 mRNA were the most significant between 0 ng/mL group and 100 ng/mL group. Its shows that the expression of T-bet mRNA and the raatio of T-bet/GATA-3 decreased, the expression of GATA-3 mRNA increased in the 100 ng/mL group. Conclusion： IL-11 in vitro can promote Thl cell convert to Th2 cell in patients with ITP, and can increase the expression of GATA-3 mRNA and decrease the expression T-bet mRNA.