| 噬菌体展示技术筛选卵巢癌细胞表面转移相关分子结合肽 |
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| 引用本文： | 李文桔,王乐丹,吕杰强. 噬菌体展示技术筛选卵巢癌细胞表面转移相关分子结合肽[J]. 温州医学院学报, 2014, 0(2): 127-129,133 |
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| 作者姓名： | 李文桔 王乐丹 吕杰强 |
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| 作者单位： | 温州医科大学附属第二医院妇科,浙江温州325027 |
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| 摘 要： | 目的：利用噬茵体7肽库筛选高转移潜能卵巢癌细胞株HO-8910PM表面转移相关分子结合肽。方法：利用噬茵体展示技术体外快速差减筛选（biopanning and rapid analysis of selective inter-activeligands，BRASIL）卵巢癌细胞株HO-8910和HO-8910PM，经过连续5轮生物淘洗，随机挑选14个噬茵体单克隆进行DNA测序，并进行ELISA、噬茵体竞争结合实验验证阳性噬茵体的亲和性。结果：噬菌体克隆Z3（短肽LRLRNTR）对高转移潜能卵巢癌细胞株HO-8910PM有较高的亲和性。结论：噬茵体展示技术筛选的短肽LRLRNTR可望为卵巢肿瘤早期诊断、转移复发及治疗提供新的方向。
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| 关 键 词： | 卵巢肿瘤 噬菌体展示技术 噬菌体多肽库 多肽 |
Screening metastasis related peptides binding to the surface of ovarian tumor cells by phage display |
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| Affiliation: | LI Wenju, WANG Ledan, L V Jieqiang. (Department of Gynecology, the Second Affiliated Hospital of Wenzhou Medical University, Wenzhou, 325027) |
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| Abstract: | Objective： Using 7-mer phage display peptide library in vitro to isolate metastasis relatedpeptides on the surface of the high metastatic potential ovarian tumor cell line HO-8910PM. Methods： Phage display technology with biopanning and rapid analysis of selective interactive ligands between cell lines HO-8910 and HO-8910PM was utilized. After five rounds of biopanning, 14 phage clones were randomly selected for sequencing of DNA. Using ELISA and phage competitive binding experiment the affinity of positive phages combined with HO-8910PM cells was identified. Results： The phage clone named Z3 （peptide LRLRNTR） showed higher affinity to HO-8910PM cells. Coneluslou： The peptide LRLRNTR screened by phage display technology may offer a new direction for early diagnosis, metastasis and treatment of ovarian tumor. |
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| Keywords: | ovarian tumor phage display technology phage peptide library peptides |
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