异丙酚对油酸性急性肺损伤大鼠肺表面活性蛋白A及粘附因子-1的影响

目的研究静脉麻醉药异丙酚对油酸性急性肺损伤(ALI)大鼠肺表面活性蛋白A(SP-A)及粘附因子-1(ICAM-1)的影响。方法将80只大鼠随机分成正常对照组、ALI组和异丙酚低(4mg/(kg·h))、中(8mg/(kg·h))、高(16mg/(kg·h))剂量治疗组。各组大鼠均于药物治疗4h后行腹主动脉放血处死,取肺组织分别进行光、电镜病理观察。测定肺湿干比、肺水含量,WesternBlot法检测支气管肺泡灌洗液(BALF)中SP-A含量,分别以免疫组化(IHC)及流式细胞术(FCM)检测肺组织中ICAM-1的表达及含量。结果电镜显示ALI组大鼠肺泡Ⅱ型上皮细胞线粒体、粗面内质网及嗜锇性板层小体损伤,低、中、高剂量治疗组肺泡Ⅱ型上皮细胞细胞器损伤均有不同程度改善。与正常对照组比较,ALI组肺组织肺湿干比、肺水含量及ICAM-1表达升高(P<0·05),BALF中SP-A含量减少(P<0·05);静脉输注异丙酚使ALI大鼠肺组织肺干湿比、肺水含量及ICAM-1表达降低,BALF中SP-A含量增加(P<0·05)。结论4～16mg/(kg·h)异丙酚均可抑制油酸性ALI时ICAM-1过度表达,增加BALF中SP-A的含量,减轻肺组织的损伤程度,对油酸性ALI起到一定的保护作用,8mg/(kg·h)剂量效果较好。

Effects of Propofol on SP - A Content and ICAM - 1 Expression in Oleic Acid - induced Acute Lung Injury Rats

OBJECTIVE:To investigate the effects of propofol on the content of SP-A(surfactant-associated protein A)and the expression of intercellular adhesion molecule-1(ICAM-1)in oleic acid-induced acute lung injury(ALI)rats.METHODS:A total of 80 rats were randomly divided into control group,ALI group,low-dose(4mg/(kg·h),medium-dose(8mg/(kg·h)and high-dose(16mg/(kg·h)propofol treatment groups.The rats in each group were put to death at 4h after administration and the lung tissues were taken for pathological observation by light microscopy and electron microscopy.Lung W/D ratio,water content in lungs were measured;the content of SP-A in bronchalveolar lavage fluid(BALF)were determined by Western Blot,and the expression of ICAM-1 in lung tissue was measured by immunohistochemistry(IHC)and flow cytometry(FCM).RESULTS:Electron microscopically,the damages of mitochondrion,rough endoplasmic reticulum and osmiophilic multi lamellar body in type Ⅱalveolar epithelial cells were noted in ALI group,and the damages of type Ⅱalveolar epithelial cells showed a different degree of amelioration in low,medium and high dose propofol treatment groups.propofol treatment groups.The expression of ICAM-1,the lung water content and lung W/D ratio were significantly increased in lung tissues while the content of SP-A in BALF were significantly decreased in ALI group as compared with control group(P<0.05).Lung W/D ratio,lung water content and ICAM-1 expression were decreased while the content of SP-A in BALF in ALI rats were increased through intravenous infusion of propofol(P<0.05).CONCLUSION:Propofol infusion at a dose of 4～16mg/(kg·h)can inhibit ICAM-1 overexpression in lung tissue with oleic acid-induced acute lung injury,increase SP-A content in BALF,lessen the degree of lung tissue injury,and it has protective effect on oleic acid-induced acute lung injury,esp.at a dose of 8 mg/(kg·h).