浆细胞膜糖蛋白-1基因与肥胖、糖尿病关系的群体水平研究

目的 研究肥胖、2型糖尿病、胰岛素抵抗与候选基因PC-1之间的关系.方法 运用PCR-RFLP技术分析53例正常对照者,105例肥胖患者,63例糖尿病非肥胖患者,114例肥胖伴糖尿病患者PC-1基因编码区K121Q多态性.结果 Q等位基因频率在正常对照、肥胖人群、糖尿病非肥胖人群、糖尿病伴肥胖人分别为3%,18%,4%和30%.与正常对照组比较,OB组、OB＋H组、OB＋I组、OB＋L组、OB＋HIL组的相对危险性（RR）分别为4.26,4.12,7.36,5.15,9.70;与单纯糖尿病组比较,DM＋OB组、DM＋OBH组、DM＋OBI组、DM＋OBL组、DM＋OBHIL组的RR分别5.32,7.37,12.07,8.53,13.50.结论 PC-1 Q等位基因与肥胖、肥胖2型糖尿病、胰岛素抵抗的发生呈正相关,它参与了部分中国人肥胖、2型糖尿病、胰岛素抵抗的发生.

Relationship between plasma membrane glycoprotein gene and obesity, type 2 diabetes mellitus, insulin resistance in Chinese population

Objective To explore the relationship between the plasma membrane glycoprotein ( PC-1 ) gene and obesity, type 2 diabetes mellitus, insulin resistance in Chinese population. Methods 53 norma1 subjects, 105 simple obesity subjects, 63 type 2 diabetic patients and 114 obesity type 2 diabetics have been genotyped with PCR-RFLP. Results The frequency of PC-1 gene Q allele was 3%, 18%, 4% and 30% in control, obesity subjects, diabetic patients and obesity diabetic individuals, respectively. Compared with control group, the relative risk (RR) in OB group, OBH grop, OBI group, OBL group and OBHIL group was 4. 26,4. 12,7. 36,5. 15 and 9. 70, respectively. Compared with diabetes group, the RR in diabetes with DMOB, diabetes with OBH group, diabetes with OBI group, diabetes with OBL group and diabetes with OBHIL group was 5.23,7. 37,12. 07,8. 53 and 13. 50, respectively. Concluision The frequency of PC-1 gene Q allele were significantly associated with obesity, obesity diabetics and insulin resistance in Chinese. The results suggested that the PC-1 gene Q allele was a potential genetic marker for obesity, type 2 diabetes and insulin resistance.