分子杂交技术对外周血单个核细胞及肝细胞内HBV—DNA的初步研究

本文分别用转移印迹杂交和斑点杂交检测61例乙肝患者外周血单个核细胞(PBMC)及血清中的HBV-DNA。同时用转移印迹杂交和原位杂交检测其中31例患者肝活检组织中的HBV-DNA。结果发现血清、PBMC及肝组织HBV-DNA阳性率分别为26.2％(16/61),24.6％(15/61)及48.4％(15/31)。同一个体内,血清、PBMC及肝组织内HBV-DNA之间无明确的关联,血清HBV-DNA阴性时,仍可在11例患者的PBMC或肝内检出HBV-DNA。结果表明,PBMC可能也是独立于肝外的HBV贮存及复制的场所。血清HBV-DNA阴性不能否定体内仍有HBV复制的可能。

Investigation of HBV-DNA in peripheral blood mononuclear cells and hepatocytes with molecular hybridization

The existence of HBV-DNA in the mononuclear cells of peripheral blood and in the serum of 61 patients with HBV infection was determined with southern blot and dot blot hybridization,and that in the liver tissue of 31 patients out of the 61 with southern blot and in situ hybridization.The positive rate of HBV-DNA in the serum,mononuclear cells and hepatocytrs was 26.2% (16/61),24.6% (15/61) and 44.8% (13/31) respectively.There was no concordance of the existence of HBV-DNA in the serum,peripheral mono-nuclear cells and hepatocytes in an individual.For example,HBV-DNA was absent in the serum but present in mononuclear cells and hepatocytes in 11 cases.In fact,peripheral mononuclsar cells can serve as the reservoir for HBV to replicate.It cannot be denied that HBV can replicate in an individual even though HBV-DNA is negative in the serum.