| 青蒿琥酯对人胚肺成纤维细胞Caspase-3表达的影响 |
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| 引用本文： | 王昌明,黎洪秀,张孝飞. 青蒿琥酯对人胚肺成纤维细胞Caspase-3表达的影响[J]. 中国现代应用药学, 2011, 28(1): 11-14 |
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| 作者姓名： | 王昌明 黎洪秀 张孝飞 |
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| 作者单位： | 桂林医学院附属医院，广西 桂林541001;桂林医学院附属医院，广西 桂林541001;桂林医学院附属医院，广西 桂林541001 |
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| 基金项目： | 广西科学研究与技术开发计划项目(桂科攻10124008-4) |
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| 摘 要： | 目的 研究青蒿琥酯对人胚肺成纤维细胞(human embryonic lung fibroblast,HELF)系HFL-Ⅰ细胞体外生长的影响,为青蒿琥酯抗纤维化提供实验依据.方法 采用CCK-8法检测青蒿琥酯对体外培养的HFL-Ⅰ细胞生长的影响,用流式细胞术测定细胞凋亡率;RT-PCR法测定凋亡相关蛋白Caspase...
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| 关 键 词： | 青蒿琥酯 人胚肺成纤维细胞 凋亡 Caspase-3 |
| 收稿时间： | 2010-06-17 |
Effect of Artesunate on the Expression of Caspase-3 in Human Embryonic Lung Fibroblasts |
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| WANG Changming,LI Hongxiu and ZHANG Xiaofei. Effect of Artesunate on the Expression of Caspase-3 in Human Embryonic Lung Fibroblasts[J]. The Chinese Journal of Modern Applied Pharmacy, 2011, 28(1): 11-14 |
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| Authors: | WANG Changming LI Hongxiu ZHANG Xiaofei |
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| Affiliation: | The Affiliated Hospital of Guilin Medical College, Guilin 541001, China;The Affiliated Hospital of Guilin Medical College, Guilin 541001, China;The Affiliated Hospital of Guilin Medical College, Guilin 541001, China |
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| Abstract: | OBJECTIVE To study the effect of artesunate on the growth of human embryonic lung fibroblast HFL-I cells in vitro and provide experimental data for anti-fibrosis activity of artesunate.METHODS CCK-8 assay was used to determine the effect of artesunate on the growth of HFL-I cells in vitro.Apoptosis ratio was examined by flow cytometry(FCM).The mRNA level of Caspase-3,one of apoptosis related proteins,were assessed by RT-PCR.The expression of Caspase-3 protein was detected by Western blot.RESULTS Artesunate ... |
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| Keywords: | artesunate human embryonic lung fibroblasts apoptosis Caspase-3 |
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