| 大鼠睾丸乳酸脱氢酶同工酶－C_4的分离纯化研究 |
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| 引用本文： | 谭委民,朱雯,丘志昌,苗秉.大鼠睾丸乳酸脱氢酶同工酶－C_4的分离纯化研究[J].南京医科大学学报,1994(2). |
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| 作者姓名： | 谭委民 朱雯 丘志昌 苗秉 |
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| 摘 要： | 采用加热、硫酸铵分段盐析和ＤＥＡＥ－ＳｅｐｈａｄｅｘＡ－５０柱层析，从大鼠睾丸中分离纯化了ＬＤＨ－Ｃ４。比活性提高７８４倍，活性回收率为１２．３％，纯化的ＬＤＨ－Ｃ４经聚丙烯酰胺凝胶电泳（ＰＡＧＥ）鉴定均为一条酶带，位于ＬＤＨ３和ＬＤＨ４之间。
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| 关 键 词： | 睾丸 LDH－C_4 纯化 大鼠 |
Isolation and Purification of Rat Testicular Lactate Dehydrogenase C_4 |
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| Tan Weimin,et al..Isolation and Purification of Rat Testicular Lactate Dehydrogenase C_4[J].Acta Universitatis Medicinalis Nanjing,1994(2). |
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| Authors: | Tan Weimin |
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| Institution: | Department of Biochemistry |
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| Abstract: | The lactate dehydrogenase isoenzyme C4 of rat testicle was purified with a yield of 12.3%and a raise of specific activity to 784-fold. The purified product was PAGE homogeneous and migrated after LDH3 during electrophoresis. |
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| Keywords: | rat testis LDH-C_4 purification |
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