白细胞介素-1及其抑制剂对眼眶成纤维细胞增殖和合成透明质酸作用的研究

目的：观察白细胞介素1(IL-1)及白细胞介素1受体拮抗剂(IL-1Ra)对甲状腺相关眼病(TAO)病人及正常人眼眶成纤维细胞(OFs)增殖及合成透明质酸(HA)的作用。方法：取5例TAO病人眼眶组织与眼外肌进行OFs培养，另取4例无眼部病变的意外死亡者眼眶组织与眼外肌进行OFs培养作为对照。分别在其中加人不同浓度的IL-1、IL-1Ra，用MTT法及放射免疫法观察它们对OFs增殖及分泌HA的作用；然后用不同浓度的IL-1Ra与IL-1共同孵育，观察OFs的增殖及HA合成的变化。结果：IL-1在5U／ml浓度以上时均能明显刺激OFs的增殖和HA的合成，当IL-1浓度为500U／ml时对TAO病人OFs的刺激作用强于对正常人OFs.IL-1Ra单独使用时对OFs的增殖及HA合成均没有显著性影响。当IL-1Ra(5、50ng／m1)与IL-1(500U／m1)联合孵育时，能阻断IL-1对OFs的刺激作用。结论：IL-1能刺激TAO病人与正常人OFs的增殖和HA的合成，而IL-1Ra能阻断其刺激作用，因此IL-lRa可能是TAO治疗中的一个新途径。

Study on Effects of Interleukin-1 and Their Inhibitors for Proliferation and Hyaluronic Acid Synthesis of Cultured Human Orbital Fibroblasts

Purpose:To observe the proliferation and synthesis of hyaluronic acid (HA) of cultured human orbital fibroblasts (OFs) affected by interleukin-1 (IL-1), and their inhibitors interleukin-1 receptor antagonist(IL-1Ra).Methods:OFs from 5 patients with thyroid-associated ophthahnopathy (TAO) and 4 normal subjects were cultured in vitro. IL-1, IL-1Ra with different concentrations were put into media respectively, and IL-1 (500 U/ml),IL-1Ra(5, 50 ng/ml) were put into media together. The proliferation and HA production of OFs were measured by MTT and radioimmunological technique.Results:IL-1 (550 500 U/ml) can significantly increase the proliferation of OFs and synthesis of HA (P<0.05), IL-I ( 500 U/ml) stimulate more proliferation of OFs and HA production of OFs from TAO patients than that of OFs from normal subjects, while IL-1Ra alone had no inhibitive effects at any coneentrations.IL1Ra (5,50 ng/ml) can block the proliferation and HA production of OFs when IL-1 with IL-1Ra incubated together.Conclusion:IL-1 can stimulate the proliferation and HA synthesis of OFs. IL-1Ra is an effective inhibitor of proliferation and synthesis of OFs induced by IL-1. IL-1Ra may be useful in the prevention or treatment of TAO. Eye Science 2003;19:232-235.