人胆管癌鸡胚移植瘤模型的建立及其生物学特性的研究

目的 建立人胆管癌鸡胚移植瘤模型，研究其形态学及生物学特性。方法 将人胆管癌细胞接种于鸡胚尿囊膜，观察影响胆管癌移植瘤成活的因素、移植瘤生长特性、形态学特征和生物学性状。结果 建立了人胆管癌鸡胚移植瘤模型。移植瘤易于生长，具有较强的血管诱导作用。光镜、电镜下移植瘤具有与人胆管癌类似的组织结构。结论 该模型可应用于胆管癌的生物学行为研究、药物筛选等领域，具有模型易于复制、操作简单、适用范围广等特点，是研究胆管癌的较好的动物模型。

Establishment of a human bile duct carcinoma model on the chick embryo chorioallantoic membrane and study of its biological properties

Objective To establish a human bile duct carcinoma model on the chick embryo chorioallantoic membrane (CAM) and study its morphological and biological properties. Methods Human bile duct carcinoma cell line QBC939 was cultivated and implanted on the CAMs. The factors affecting the implanted tumors and the morphological and biological properties of the tumors were studied. Results A human bile duct carcinoma model on the CAM was established. The implanted tumors, easy to grow on the CAM, had a strong trend of angiogensis and similar structure as human bile duct carcinoma with microscopic verification. Conclusion Our findings suggest that this model is of easy duplication and simple operation, and can be used in the research of the biological properties and drug selection for human bile duct carcinoma.