| HPLC法测定花锚中落干酸和龙胆苦苷的含量 |
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| 引用本文： | 俞青芬.HPLC法测定花锚中落干酸和龙胆苦苷的含量[J].微量元素与健康研究,2007,24(1):42-43. |
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| 作者姓名： | 俞青芬 |
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| 作者单位： | 青海民族学院化学系,西宁,810007 |
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| 摘 要： | 目的:建立反高效液相色谱法测定花锚中落干酸和龙胆苦苷的含量。方法:采用kromasil—C18(250mm×4.6mm,5μm)色谱柱,以流动相甲醇和水(含0.04%磷酸)的比例在0~15min内由23:77至30:70线性梯度洗脱,流速1 ml/min,检测波长238 nm,柱温35℃。结果:两种成分均达到基线分离,落干酸和龙胆苦苷的线性范围分别为0.198~5.544μg(r=0.9999),0.0262~0.7336μg(r=0.9999);回收率为96.12%(RSD=1.73%)、97.67%(RSD=1.91%)。结论:方法测定快速,结果准确、可靠。
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| 关 键 词： | 反相高效液相色谱 花锚 落干酸 龙胆苦苷 |
| 文章编号： | 1005-5320(2007)01-0042-02 |
| 修稿时间： | 2006-08-30 |
Simultaneous Determination of Loganic acid and Gentianaceae in Herba Haleniae by HPLC |
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| YU Qing-fen.Simultaneous Determination of Loganic acid and Gentianaceae in Herba Haleniae by HPLC[J].Studies of Trace Elements and Health,2007,24(1):42-43. |
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| Authors: | YU Qing-fen |
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| Institution: | Chemistry Department of Qinghai nationalities, Xining 810007, China |
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| Abstract: | Objective:To establish a quantitative method of simultaneously determination of loganic acid and gentiopicroside by RP—HPLC.Method:The sample wee separated on the column of kromasil—C_(18)(250mm×4.6mm,5μm)which eluted with methanol and water(0.04% phosphoric acid).The ratio of methanol and water increased from 23:77 to 30:70 in 15 min with detected wavelength at 238nm,flow rate at 1ml/min,column temperature at 350C.Results:Two compounds were baseisolated.The linear ranges of loganic acid,gentiopicroside were 0.198～5.544 μg(r=0.9999),0.0262～0.7336μg(r=0.9999);The average recoveries were 96.12%(RSD=1.73%)、97.67%(RSD=1.91%).Conclusions:The method is rapid and precise. |
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| Keywords: | RP--HPLC Herba Haleniae loganic acid gentiopicroside |
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