静息态功能磁共振评价针刺足三里穴后效应

目的 研究针刺足三里穴后引起静息态脑功能活动的时间变化规律,探讨针刺后效应.方法 对17名健康志愿者,采集针刺双侧足三里穴拔针后的第10、25、45和60 min的静息态功能磁共振数据,利用REST软件计算低频振幅(ALFF),然后用SPM5与针刺前相应数据进行配对t检验,得到拔针后上述4个时间点相对于针刺前脑区ALFF的变化及其趋势.结果 针刺足三里穴拔针后引起了脑部ALFF变化,拔针后第10和第25 min ALFF显著增高脑区数量最多,主要包括双侧枕叶、双侧颞中回、左侧楔前叶、左侧顶下小叶、左侧中央后回、左侧小脑后叶、右侧扣带前回及右侧扣带后回等;拔针后第45和第60 min ALFF显著增高脑区逐渐减少,主要包括双侧枕叶、左侧楔前叶、左侧顶下小叶、左侧顶上小叶及右侧扣带后回等.结论 针刺后效应对静息态脑网络活动产生显著影响,拔针后第10和25 min为针刺后效应重要时间点.

Resting-state functional MRI evaluation of after-effect of acupuncture at Zusanli point

Objective To investigate time related changes in resting-state activity induced by acupuncture at Zusanli point with resting-state functional MRI (fMRI). Methods Seventeen healthy volunteers were acupunctured separately at Zusanli point (ST 36, bilateral legs) in this study. The resting-state fMRI data was acquired before and at 10, 25, 45 and 60 min after acupuncture. Paired t-test was used to analyze the changes of amplitude of low-frequency fluctuation (ALFF) of resting-state brain activity between the pre- and post-acupuncture of the each timepoint. Results ALFF increased significantly reached maximum at 10 and 25 min, including bilateral occipital lobe, bilateral temporal lobe, left precuneus, left inferior parietal lobule, left postcentral gyrus, left cerebellum posterior lobe, right anterior and posterior cingutate gyrus. ALFF decreased gradually at 45 and 60 min after acupuncture, including bilateral occipital lobe, left precuneus, left inferior parietal lobule, left superior parietal lobule and right posterior cingutate gyrus. Conclusion Acupuncture at Zusanli point can influence the resting-state brain activity conspicuously, and the after-effect of acupuncture is most remarkable at 10 min and 25 min after acupuncture.