苯环利定对兔急性脑缺血损伤的保护作用——脑脊液中β-内啡肽免疫活性分析

结扎兔双侧椎动脉和颈总动脉,导致急性脑缺血。缺血30min后重新开放双侧颈总动脉,使再灌注。脑缺血30min再灌注2h后,脑脊液中β—内啡肽免疫活性物质含量比缺血前增加1.01±0.52mg/L。icv苯环利定20μg/kg组,脑脊液中β—内啡肽免疫活性物质含量比缺血前增加0.36±0.32mg/L。icv苯环利定80μg/kg组,脑脊液中β—内啡肽免疫活性物质含量比缺血前下降0.19±0.44mg/L。提示苯环利定能抑制脑缺血后脑内β—内啡肽释放,减轻脑缺血性神经损伤。

PROTECTIVE EFFECT OF PHENCYCLIDINE ON ACUTE CEREBRAL ISCHEMIA OF RABBIT-ANALYSIS OF BETA- ENDORPHIN IMMUNOREACTIVITY IN CEREBROSPINAL FLUID

Acute cerebral ischemia and reperfusion injury of rabbits was produced by permanently occluding the vertebral arteries and temporarily clamping the common carotid arteries for 30 min. Beta-endorphin immunoreactive substance ( ir beta-endorphin ) content were measured in cerebrospinal fluid at pre-ischemia and after ischemia and reperfusion stages. In control group, the content of ir beta-endorphin significantly increased (1.01±0.52 mg/L, P< 0.05). In phencyclidine 20μg/kg icv treated group, the content of ir beta-endorphin increased slightly ( 0.36 ± 0.32 mg/L) during ischemia and reperfusion, however in phencyclidine 80μg/kg icv treated group, the content or ir beta-endorphin decreased (0.19±0.44 mg/L), which was significant difference from control group ( P<0.05 ). These results indicate that phencyclidine may antagonize the centrally released beta-endorphin induced by acute cerebral ischemia and show benefical effects in cerebral ischemic damage.