莲心碱对血管平滑肌细胞增殖及热应激蛋白70和P53表达的影响

采用培养的内皮素所致血管平滑肌细胞增殖模型。用氚-胸腺嘧啶核苷掺入法、流式细胞术、Westernblot及Northernblot分析方法，观察了莲心减对血管平滑肌细胞增殖的作用及对热应激蛋白70及其mRNA和抑癌基因P53表达的影响。结果发现，遂心碱能逆转内皮素所致的~3H－TdR掺入量增多，阻止血管平滑肌细胞由Go／G1期进入DNA合成期（S期）和有丝分裂期（G2／M期）．并能使效应激蛋白70及mRNA表达减弱，P53抑癌基因及mRNA表达增强。以上结果提示，莲心碱能抑制血管平滑肌细胞增殖，这可能与热应激蛋白70及P53的调控有关。

Effects of Liensinine on Proliferation of Vascular Smooth Muscle Cells and Heat Stress Protein 70 and P53 Expression

Aim To determine the effects of Liensinine on endothelin-stimulated proliferation of vascular smooth muscle cells (VSMC) and expression of heat stress protein (HSP70) and antioncogene P53. Metbods The experimental models of proliferation of cultured porcine aortic smooth muscle cells induced by endothelin were established, and H-thymidine(~3 H-TdR ) incorporation, flow cytometry, Western blot and Northern blot analysis were used.Rerults Liensinine may drop H-TdR incorporation induced by endotbelin and hold-back VSMC from G0/ G, phase to DNA synthetic phase (S) and mitotic phase (G2/M ). Furthermore, Liensinine could reverse the enkanced expression of HSP70 and HSP70 mRNA and reduced expression of antioncogene P53 induced by endothelin.Conclusions Liensinine may inhibit DNA synthesis and proliferation of VSMC, related to the mechanism of molecular biology of controlling HSP70 and P53.