丙丁酚对氧化型低密度脂蛋白损伤内皮的保护作用

用培养的人脐静脉内皮细胞为对象，用~3H-TdR掺入法，观察了抗氧化剂丙丁酚对氧化型低密度脂蛋白作用下的内皮细胞DNA合成的保护作用，并用扫描电子显微镜观察其对培养细胞形态结构的影响．结果发现，在作用24h后，氧化型低密度脂蛋白组的~3H－TdR掺入量是天然低密度脂蛋白组的48％，是丙丁酚组的56％（P＜0．001），超微结构显示丙丁酚组的细胞结构保持正常，而氧化型低密度脂蛋白组则受到破坏．本实验显示丙丁酚对内皮细胞的氧自由基损伤有明显的保护作用．

Probucol Prevents the Cultured Endotbelial Cells from Injury Induced by Oxidized Low Density Lipoprotein

Aim Endothelial cells injured by free radicals is a crucial step in tbe development of many diseases. We investigated the protective effect of probucol. an an-tiatherosclerosis drug,to the cultured hurnan endothe-lial cells.Methods The oxidized low density lipoprotein (OLDL) were used as free radial resources and 'H-TdR incorporation of the cells was estimated as DNA sgnthesis. The microstructure change was studied by using the scanning electron misroscope.Results At 24 hours interval, the k-TdR incorPo-rated into the cells in OLDL group was 48K as that of nLDL group or 56K as that of probucol OLDL group (P<0.001 ). The scanning electron micro-scopic study showed that the intercellular junctions di-ndnished and the cellular outline changed in the OLDL treated cells but not the probucol treated cells.CoarIasloas Probucol is an useful antioxidant in preventing the cultured cells from injury by free radi-cals and perhaps tbus contributes to the antiathero-genesis effeCt.