API0134对血管平滑肌细胞增殖和血小板源生长因子B链、碱性纤维母细胞生长因子及相关癌基因表达的影响

为探讨ＡＰＩ０１３４防治冠状动脉继样硬化和腔内成形术后再狭窄的作用机制，采用内皮素诱导建立培养的血管平滑肌细胞增殖模型，用氚标胸腺嘧啶脱氧核苷掺入法，流式细胞术、免疫细胞化学检测及Ｎｏｒｔｈｅｒｎｂｌｏｔ方法，观察了ＡＰＩ０１３４对血管平滑肌细胞增殖的作用及对血小板源生长因子Ｂ链、碱性纤维母细胞生长因子及其相关癌基因ｃ－ｓｉｓ和ｃ－ｍｙｃ表达的影响。结果发现，ＡＰＩ０１３４能逆转内皮素所致的氚标胸

Effects of API0134 on Proliferation of Vascular Smooth Muscle Cells and Expression of Platelet Derived Growth Factor-B Chain, Basic Fibroblast Growth Factor, c-sis and c-myc

Aim To determine the effects of API0l34 on en-dothelin (ET)-stimulated proliferation of vascularsmooth muscle cells (VSMC ) and expression ofgrowth factor, such as platelet derived growth factor(PDGF-B ) and basic fibrob1ast growth factor(bFGF), and oncogene c-sis and c-myc.Methods The experimental models of proliferationof cultured porcine aortic smooth muscle cells inducedby ET were established, and 3H-thymidine (3H-TdR ) incorporation, flow cytometry, immunohistochemistry and Northern blot assaies were used.Results API0134 may drop 3H-TdR data in-creased by ET and hold-back VSMC from static phase(G,/G, ) to DNA synthetic phase (S) and mitoticphase (G2/M ). Furthermore, API0134 could re-verse the enhanced expression of antigen PDGF-B,bFGF and reduced expression of oncogene c-sis and c-myc induced by ET.Conclusion API0134 might inhibit DNA synthesisand proliferation of VSMC, related to the mechanismof molecular biology of controlling growth factors andoncogene.