FHIT基因在原发性皮肤T细胞淋巴瘤中的异常改变及意义

目的检测原发性皮肤T细胞淋巴瘤中脆性组氨酸三联体(FHIT)基因的转录情况。方法用PCR-SS-CP法检测了10例原发性皮肤T细胞淋巴瘤患者皮损FHIT基因外显子5和8的缺失和突变状况,10例健康皮肤组织做对照。结果10例肿瘤组织中,FHIT基因外显子5缺失者7例,FHIT基因外显子8的缺失者2例;而正常人FHIT基因外显子5和8无1例缺失;对扩出的外显子进行SSCP分析,未检测到其突变。结论原发性皮肤T细胞淋巴瘤中存在FHIT基因外显子5和8的异常缺失,这种异常转录可能与该病的发生有关。

Detection and Significance of Abnormality FHIT Gene Exons in Cutis Primary T Cell Lymphoma

Objective To detect deletion and mutation of exon 5 and 8 of FHIT gene in cutis primary T cell lymphoma,and to analyze the role of the abnormality in the carcinogenesis of cutis cancer.Methods The deletion and mutations of exon5,8 of FHIT gene were detected in 10 cutis primary T cell lymphoma samples tissues by polymerase chain reaction and single-strand conformation polymorphism(PCR-SSCP)methods.Results Deletion of exon 5 was observed in 7 out of 10 lymphoma samples,and the deletion of exon 8 was 2 out of 10 lymphoma.No mutation was found in exon 5,8 of FHIT gene.Conclusion Deletion of exon 5,8 was observed in cutis primary T cell lymphoma and no mutation was found.The abnormality may play a role in the pathogenesis of the cutis primary T cell lymphoma。