大黄炮制前后物质基础变化研究

目的:探讨大黄不同炮制品在炮制前后物质基础的变化规律。方法:分别制备生大黄、酒大黄、熟大黄、大黄炭等几种炮制品,采用薄层色谱法测定不同炮制品中干燥失质量和酸不溶成分、总灰分、浸出物含量;并测定鞣质及蒽醌含量变化。结果:生大黄、酒大黄、熟大黄、大黄炭总灰分及酸不溶灰分成分含量相差不多;酒大黄及熟大黄干燥失质量及浸出物含量没有明显变化,大黄炭干燥失质量及浸出物含量明显降低;大黄在炮制前后蒽醌及鞣质含量发生了变化,且变化程度同炮制条件的强烈程度相关。熟大黄中游离蒽醌含量最高,生大黄中结合蒽醌含量最多。结论:鞣质及蒽醌类物质为大黄泻下解热的物质基础。对饮片不同炮制品的物质基础变化进行研究,可为不同炮制品的质量鉴定提供参考。

Change of Material Base of Rheum palmatum before and after Processing

OBJECTIVE ： To probe into the change regularity of material base for different specifications of Rheum palmatum decoction pieces before and after processing. METHODS：R. palmatum, prepared R. palmatum with wine, prepared R. palmatum, charred R. palmatum and other processed preparation were preparation respectively; loss on drying, the contents of acid insoluble components, total ash content and extract in different processed R. palmatum products were all determined by TLC. Also, the con- tents of tannin and anthraquinone were determined. RESULTS： It was similar that the contents of total ash and acid insoluble com- ponents in R. palmatum, prepared R. palmatum with wine, prepared R. palmatum, charred R. palmatum; no obvious change was found in the loss on drying and the content of extract from prepared R. palmatum with wine and prepared R. palmatum; the loss on drying and the content of extract from charred R, palmatum decreased significantly. The contents of tannin and anthraquinone in R. palmatum before and after processing had changes, which were related with the density of process technology. The content of free anthraquinone in prepared R. palmatum was the most, and the content of anthraquinone in R. palmatum was the most. CONCLU- SIONS： Tannin and anthraquinone were the material base for purgation and fever relief. The research on the changes of material base for different processed products can provide reference for the quality identification of different processed products.