顺铂诱导颊癌细胞凋亡及凋亡分子(Apo-1)表达的实验研究

目的:研究细胞毒类化疗药——顺铂诱导人颊癌细胞凋亡发生的规律以及凋亡分子Apo-1的表达。探讨口腔癌化疗的细胞凋亡机制。方法:以建株人颊癌细胞系BcaCD885为对象,采用光镜、电镜以及流式细胞术等手段观察顺铂诱导颊癌细胞凋亡的形态特征及规律,同时检测顺铂对颊癌细胞Apo-1表达的影响。结果:顺铂作用后的颊癌细胞出现典型的凋亡细胞形态学特征;顺铂诱导颊癌细胞凋亡呈现时间及剂量依赖性效应关系;顺铂能上调颊癌细胞Apo-1的表达。结论:细胞凋亡可能是顺铂杀灭颊癌细胞的重要机制,Apo-1在顺铂诱导颊癌细胞凋亡中可能起着关键性调节作用

Experimental Study on Apoptosis and Apo 1 Expression \;of Buccal Carcinoma Cell(BCC) Induced by Cisplatin

OBJECTIVE: To study apoptotic characteristics and expression of apoptosis molecule Apo-1 (Fas, CD95) on buccal carcinoma cell (BCC) induced by cytotoxic drug cisplatin and exploit the apoptotic mechanisms of oral cancer chemotherapy. METHODS: Human BCC line BcaCD885 was used as in vitro study object. Morphological features of apoptotic BCC induced by cisplatin were observed by use of lightmicroscopy and electronmicroscopy, meanwhile, apoptotic rates (AR) were analysed by the flow cytometry. RESULTS: 1. Condensation and crack of nucleus and apoptotic bodies appeared in apoptotic BCC; 2. There existed time and dosage dependent AR, and AR were respectively 5.4%, 7.4%, 9.4%, 20.3% and 22.6% after BCC had been acted by 15 micrograms/ml cisplatin for 2, 6, 12 and 24 hours, moreover, there was significantly difference (P < 0.01) among the 12-hour group, the 24-hour group and the control group; 3. Cell cycle of BCC was apparently arrested at G0/G1 by cisplatin (P < 0.05); 4. Apo-1 expression of BCC were enhanced 58% and 62% respectively after it was induced by 25 micrograms/ml cisplatin for 18 and 42 hours (P < 0.05). CONCLUSION: Apoptosis may be an important mechanism of BCC killed by cisplatin, and Apo-1 may play a role in the process of apoptosis induced by chemotherapy drugs.