尿毒症血液透析患者T淋巴细胞亚群的变化

目的了解尿毒症血液透析患者T淋巴细胞亚群改变致肿瘤易感性。方法过氧化物酶标记的碱性磷酸酶染色（SAP）法检测对照组、尿毒症未透析组及尿毒症透析组患者透析前、后外周血单个核细胞中CD3＋、CD4＋、CD8＋百分比的变化。结果尿毒症未透析患者外周血T细胞亚群百分比CD3＋（58.90±2.10）%、CD4＋（34.70±1.70）%、CD8＋（24.80±1.30）%明显低于正常对照组CD3＋（68.17±4.70）%、CD4＋（41.82±3.15）%、CD8＋（28.90±2.10）%（P〈0.05）;尿毒症透析组,透析前（1.00±0.10）,透析后（1.03±0.16）CD4＋/CD8＋比值,显著低于正常对照组（1.45±0.18）（P〈0.05）。尿毒症血透组患者透析后与透析前比较,CD3＋、CD4＋、CD8＋百分比和CD4＋/CD8＋比值差异均无统计学意义（P〉0.05）。结论尿毒症未透析患者T细胞免疫功能受抑制,血液透析过程未能有效地改善尿毒症患者T细胞免疫功能,增加了血液透析患者肿瘤的易感性。

T lymphocyte subsets changes in uremic hemodialysis patients

Objective To investigate the tumor susceptibility of T lymphocyte subsets changes in hemodialysis patients.Methods Strepta-vidin/alkaline phosphatase（SAP） method was used to detect peripheral blood mononuclear cells CD3＋,CD4＋,CD8＋ percentage change in normal controls,uremia non-dialysis patients and hemodialysis patients pre and post HD process.Results For uremia non-dialysis patients,peripheral blood cells T lymphocyte subsets CD3＋（58.90±2.10）%,CD4＋（34.70±1.70）%,CD8＋（24.80±1.30）% were obviously lower than those of normal contrapositive group,CD3＋（68.17±4.70）%,CD4＋（41.82±3.15）%,CD8＋（28.90±2.10）%（P0.05）,and the CD4＋/CD8＋ ratio of before trans-analysis（1.00±0.10）,after trans-analysis（1.03±0.16） were very significantly lower than that normal controls（1.45±0.18）（P0.05）.The peripheral blood cells T lymphocyte subsets CD3＋,CD4＋,CD8＋ and the CD4＋/CD8＋ ratio did not vary almost from beginning of trans-analysis to end.Conclusion T-cell immune function got inhibition in uremia non-dialysis patients,hemodialysis process failed to improve the immune function of T cells in uremia patients effectually,the tumor susceptibility of hemodialysis patients of uremia is higher than normal contrapositive group.