同型半胱氨酸对血管生成的影响及叶酸的作用 |
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引用本文: | 陈宝丽,潘达亮,罗敏虹,张鑫. 同型半胱氨酸对血管生成的影响及叶酸的作用[J]. 临床肾脏病杂志, 2013, 13(8): 375-379 |
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作者姓名: | 陈宝丽 潘达亮 罗敏虹 张鑫 |
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作者单位: | 1. 深圳市第四(福田)人民医院肾内科,深圳,518033 2. 广东医学院基础学院 |
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基金项目: | 深圳市福田区卫生公益性科研项目 |
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摘 要: | 目的通过研究不同浓度同型半胱氨酸(homocysteine,HCY)对人脐静脉内皮细胞血管生成的影响及叶酸(folicacid,FA)对其的保护作用。方法实验分6组,0、1、10、100μmol/L同型半胱氨酸,10μmol/L同型半胱氨酸+100μmol/L叶酸,100μmol/L叶酸,进行体外血管生成能力实验,Transwell迁移能力检测。Western-blot检查血管生成及迁移相关蛋白表达。结果同型半胱氨酸能明显抑制血管生长因子的表达,降低人脐静脉内皮细胞的成血管环能力。同型半胱氨酸能明显抑制迁移相关蛋白表达,降低人脐静脉内皮细胞的迁移运动能力。叶酸能改善同型半胱氨酸的抑制作用。结论同型半胱氨酸通过抑制血管生长因子、ROCKl/2的表达,从而抑制血管内皮细胞分裂增殖、血管环生成及迁移能力,导致内皮功能障碍,而叶酸能改善这种抑制作用。
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关 键 词: | 半胱氨酸 动脉粥样硬化 叶酸 血管生成 内皮功能紊乱 |
Homocysteine effects on angiogenesis and the role of folic acid |
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Affiliation: | CHEN Bao-li, PAN Da-liang, LUO Min-hong , et al. Department of Nephrology , Shenzhen 42 People ' s Hospital, Shenzhen Futian Peo- ple ' s Hospital, Shenzhen 518033, China |
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Abstract: | Objective To investigate the protective effects of folic acid(FA) on homocysteine (HCY)-induced angiogenesis activity in human umbilical vein endothelial cells(HUVECs). Methods HUVECs were divided into six groups: treatment groups(0, 1,10,and 100 μmol/L) HCY and FA pre- treatment groups(FA 100 μmol/L),and HCY 10μmol/L+ FA 100 μmol/L. HUVECs in vitro anglo- genesis kit, and migration were assayed by transwell kit. The VEGF, VEGFR2, ROCK1, ROCK2, MMP9 protein expression were measured by Western-blot analysis. Results HCY does-dependently impaired the in vitro angiogenesis and migration capacity of HUVECs, because of HCY caused a signif- icant downregulation of VEGF, ROCK1, ROCK2 protein expression. These effects of HCY on HU- VECs were reversed by FA. Conclusions Homocysteine-induced dysfunction in Endothelial dysfunc- tion(ED), which may be casued by HCY effects on downregulation of VEGF, ROCK1, ROCK2 protein expression,impaired the proliferation, and in vitro Angiogenesis capacity of HUVECs, while FA can improve this inhibition. |
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Keywords: | Cysteine Atherosclerosis Folic acid Angiogenesis Endothelial dysfunction |
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