| 神经上皮肿瘤中端粒酶活性及其调节 |
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| 引用本文： | You Y,Pu P,Peng Q,Xia Z,Huang Q,Wang C,Wang G. 神经上皮肿瘤中端粒酶活性及其调节[J]. 中华外科杂志, 2002, 40(2): 90-93 |
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| 作者姓名： | You Y Pu P Peng Q Xia Z Huang Q Wang C Wang G |
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| 作者单位： | 1. 300052,天津医科大学总医院神经外科;中国人民解放军第二五四医院神经外科
2. 300052,天津医科大学总医院神经外科 |
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| 摘 要： | 目的 研究神经上皮肿瘤的端粒酶活性及其RNA和人端粒酶逆转录酶（h TERT)的表达水平,为其临床诊断和治疗开拓思路。方法 改良端粒重复序列扩增法及RT－PCR法检测65例神经上皮肿瘤患者肿瘤标本的端粒酶活性及其RNA和hTERT表达水平,并与8例正常脑组织的标本进行对照。结果 神经上皮肿瘤端粒酶阳性率为61．5％（分别r＝0．607,r＝0．678,均P＜0．01）。结论 端粒酶活性及hTERT与神经上皮肿瘤恶性程度有关;hTERT是调节端粒酶活性的关键酶。
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| 关 键 词： | 神经上皮肿瘤 基因表达调控 酶学 端粒酶 hTERT |
| 修稿时间： | 2001-08-08 |
Telomerase activity and regulation in human neuroepithelial tumors |
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| You Yongping,Pu Peiyu,Peng Qiong,Xia Zhibo,Huang Qiang,Wang Chunyan,Wang Guangxiu. Telomerase activity and regulation in human neuroepithelial tumors[J]. Chinese Journal of Surgery, 2002, 40(2): 90-93 |
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| Authors: | You Yongping Pu Peiyu Peng Qiong Xia Zhibo Huang Qiang Wang Chunyan Wang Guangxiu |
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| Affiliation: | Department of Neurosurgery, Gereral Hospital, Tianjin Medical University, Tianjin, 300052, China. |
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| Abstract: | Objective To investigate telomerase activity and expression of hTR and hTERT in human neuroepithelial tumors for exploring new strategy for clinical diagnosis and treatment. Methods Telomerase activity was detected by modified TRAP method and the expression of hTR and hTERT was measured by RT PCR method in 65 human neuroepithelial tumors, respectively. Results The positive rates of telomerase and hTERT were 61 5% and 70 8% respectively in human neuroepithelial tumors,and the positive rate and their level of expression were correlated with the degree of malignancy of tumors positively. Conclusions Telomerase activity and hTERT are significantly correlated with the degree of malignancy in human neuroepithelial tumors. hTERT may play a key role in the regulation of telomerase activity. |
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| Keywords: | Neoplasms neuroepithelial Gene expression regulation enzymologic Telomerase |
| 本文献已被 CNKI 维普 万方数据 PubMed 等数据库收录！ |
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